Characterization of a novel peripheral nervous system myelin protein (PMP-22/SR13).
Characterization of a novel peripheral nervous system myelin protein (PMP-22/SR13).
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新型周围神经系统髓磷脂蛋白的表征（PMP-22/SR13）。
DOI：
10.1083/jcb.117.1.225
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发表时间：
1992-04
影响因子：
7.8
通讯作者：
Shooter, E M
中科院分区：
文献类型：
--
作者：
Snipes, G J;Suter, U;Welcher, A A;Shooter, E M
我们最近描述了一种新的cDNA，SR 13（Welcher，A.一、联合Suter，M.德莱昂，G. J. Snipes和E. M.枪手1991. Proc. Natl. Acad. Sci. USA. 88：7195-7199），其在坐骨神经挤压损伤后被抑制，并显示与生长停滞特异性mRNA gas 3（Manfioletti，G.，M. E.鲁阿罗湾德尔萨尔湖Philipson和C.施耐德，1990年。摩尔Cell Biol.10：2924-2930）和髓磷脂蛋白PASII（Kitamura，K.，M. Suzuki和K.上村1976.生物化学生物Biophys. Acta. 455：806-816）。在这份报告中，我们表明，22-kD SR 13蛋白表达在周围神经系统中的基本上所有有髓纤维的紧凑部分。尽管在中枢神经系统中发现了SR 13 mRNA，但通过免疫印迹分析或免疫组织化学法均未检测到相应的SR 13蛋白。周围神经系统发育过程中SR 13 mRNA和蛋白表达的北方和免疫印迹分析揭示了与其他髓鞘蛋白相似的模式。此外，我们表明，通过原位mRNA杂交的组织切片和单个神经纤维，SR 13 mRNA主要由雪旺细胞产生。我们的结论是，SR 13蛋白显然只在周围神经系统中表达，它是髓鞘的主要成分。因此，我们建议将先前指定为PASII、SR 13和gas 3的蛋白质和cDNA命名为外周髓鞘蛋白-22（PMP-22）。
We have recently described a novel cDNA, SR13 (Welcher, A. A., U. Suter, M. De Leon, G. J. Snipes, and E. M. Shooter. 1991. Proc. Natl. Acad. Sci. USA. 88:7195-7199), that is repressed after sciatic nerve crush injury and shows homology to both the growth arrest-specific mRNA, gas3 (Manfioletti, G., M. E. Ruaro, G. Del Sal, L. Philipson, and C. Schneider, 1990. Mol. Cell Biol. 10:2924-2930), and to the myelin protein, PASII (Kitamura, K., M. Suzuki, and K. Uyemura. 1976. Biochim. Biophys. Acta. 455:806-816). In this report, we show that the 22-kD SR13 protein is expressed in the compact portion of essentially all myelinated fibers in the peripheral nervous system. Although SR13 mRNA was found in the central nervous system, no corresponding SR13 protein could be detected by either immunoblot analysis or by immunohistochemistry. Northern and immunoblot analysis of SR13 mRNA and protein expression during development of the peripheral nervous system reveal a pattern similar to other myelin proteins. Furthermore, we demonstrate by in situ mRNA hybridization on tissue sections and on individual nerve fibers that SR13 mRNA is produced predominantly by Schwann cells. We conclude that the SR13 protein is apparently exclusively expressed in the peripheral nervous system where it is a major component of myelin. Thus, we propose the name Peripheral Myelin Protein-22 (PMP-22) for the proteins and cDNA previously designated PASII, SR13, and gas3.