Use of Trialkyl Ammonium Phosphate (TAAP) Buffers in Reverse Phase HPLC for High Resolution and High Recovery of Peptides and Proteins
Use of Trialkyl Ammonium Phosphate (TAAP) Buffers in Reverse Phase HPLC for High Resolution and High Recovery of Peptides and Proteins
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在反相 HPLC 中使用三烷基磷酸铵 (TAAP) 缓冲液实现肽和蛋白质的高分辨率和高回收率
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发表时间：
1978
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通讯作者：
J. Rivier
中科院分区：
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作者：
J. Rivier
本文介绍了一种与反相高效液相色谱（RP-HPLC）柱填料相容的新的缓冲体系，该体系对多肽和蛋白质具有高回收率和高分离度。TEAP缓冲液（磷酸三乙铵）是与RP-HPLC兼容的几种TAAP缓冲液（磷酸三烷基铵）之一，可为密切相关的肽或蛋白质提供高分辨率和高回收率以及日常重现性。它对紫外线透明，波长小于200 nm，用途非常广泛，可能用于其他几类化合物（例如磷脂酰胆碱和鞘磷脂）。此外，该系统在除去有机溶剂后与体外和体内生物系统相容。我们已经将我们的系统应用于放射性肽的纯化，即从密切相关的放射性杂质中分离和完全回收氚化促黄体生成素释放因子（LRF）。肽和蛋白质与LRF一样不同，...
Abstract A new buffer system compatible with reverse phase high pressure liquid chromatography (RP -HPLC) column packings and giving high recoveries and high resolution for peptides and proteins is described. The TEAP buffer (triethylammonium phosphate), one of several TAAP buffers (trialkylammonium phosphate) compatible with RP-HPLC, gives high resolution and high recovery for closely related peptides or proteins as well as day to day reproducibility. It is UV transparent to less than 200 nm, extremely versatile, and could probably be used for several other classes of compounds (phosphatidyl cholines and sphingomyelin, for example). Furthermore, this system is compatible with in vitro and in vivo biological systems after removal of the organic solvent. We have applied our system to the purification of radioactive peptides, i.e. the separation and total recovery of tritiated luteinizing hormone releasing factor (LRF) from closely related radioactive impurities. Peptides and proteins as different as LRF, s...