Tumor dormancy and cell signaling: anti-mu-induced apoptosis in human B-lymphoma cells is not caused by an APO-1-APO-1 ligand interaction.
Tumor dormancy and cell signaling: anti-mu-induced apoptosis in human B-lymphoma cells is not caused by an APO-1-APO-1 ligand interaction.
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肿瘤休眠和细胞信号转导：抗 mu 诱导的人 B 淋巴瘤细胞凋亡不是由 APO-1-APO-1 配体相互作用引起的。
DOI：
10.1073/pnas.93.5.2165
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发表时间：
1996
影响因子：
11.1
通讯作者：
Uhr,JW
中科院分区：
文献类型：
--
作者：
Racila,E;Hsueh,R;Marches,R;Tucker,TF;Krammer,PH;Scheuermann,RH;Uhr,JW
T和B淋巴瘤细胞表面抗原特异性受体的交联性启动的信号转导可诱导细胞凋亡。在T淋巴瘤细胞中，这种交联会导致APO-1配体的上调，然后APO-1配体与诱导或结构性表达的APO-1相互作用，从而触发细胞凋亡。在这里，我们表明，交联人淋巴瘤细胞(Daudi)上的膜免疫球蛋白(结构性表达APO-1)不会诱导APO-1配体的合成。此外，抗APO-1抗体的非细胞毒性片段可以阻断T细胞受体介导的T淋巴瘤细胞的凋亡，但不能阻断抗MU诱导的凋亡。因此，在B淋巴瘤细胞中，通过膜IgM信号诱导的凋亡不是由APO-1配体介导的。
Signal transduction initiated by crosslinking of antigen-specific receptors on T- and B-lymphoma cells induces apoptosis. In T-lymphoma cells, such crosslinking results in upregulation of the APO-1 ligand, which then interacts with induced or constitutively expressed APO-1, thereby triggering apoptosis. Here we show that crosslinking the membrane immunoglobulin on human lymphoma cells (Daudi) (that constitutively express APO-1) does not induce synthesis of APO-1 ligand. Further, a noncytotoxic fragment of anti-APO-1 antibody that blocks T-cell-receptor-mediated apoptosis in T-lymphoma cells does not block anti-mu-induced apoptosis. Hence, in B-lymphoma cells, apoptosis induced by signaling via membrane IgM is not mediated by the APO-1 ligand.