Chd9 mediates highly loosened chromatin structure in growing mouse oocytes
Chd9 mediates highly loosened chromatin structure in growing mouse oocytes
复制标题
DOI：
10.1016/j.bbrc.2018.04.105
复制
发表时间：
2018-06-07
影响因子：
3.1
通讯作者：
Aoki, Fugaku
中科院分区：
文献类型：
--
作者：
Ooga, Masatoshi;Funaya, Satoshi;Aoki, Fugaku
在卵子发生过程中，卵母细胞为受精后的胚胎发育做准备。这一过程背后的机制仍然是未知的。最近，有研究表明，疏松的染色质结构分别与胚胎干细胞和早期植入前胚胎的多能性和全能性有关。在这里，我们探讨了染色质松散的卵母细胞的荧光恢复后光漂白（FRAP）使用增强的绿色荧光蛋白标记的组蛋白H2 B。结果表明，生长中的卵母细胞的染色质已经高度疏松到与早期植入前胚胎相当的水平。为了阐明卵母细胞中染色质结构松散的机制，我们将重点放在染色体结构域解旋酶DNA结合蛋白9（Chd 9），这是在生长的卵母细胞中高度表达。来自使用CRISPR/Cas9系统产生的Chd 9敲除小鼠（Chd 9（-/-））的卵母细胞表现出比野生型小鼠更不松散的染色质结构，这表明Chd 9参与了生长中的卵母细胞中松散的染色质结构。这些结果表明，一个松散的染色质结构，这是由Chd 9介导的，是获得全能性受精后的先决条件。(C)2018爱思唯尔公司All rights reserved.
During oogenesis, oocytes prepare for embryonic development following fertilization. The mechanisms underlying this process are still unknown. Recently, it has been suggested that a loosened chromatin structure is involved in pluripotency and totipotency in embryonic stem (ES) cells and early preimplantation embryos, respectively. Here, we explored chromatin looseness in oocytes by fluorescence recovery after photobleaching (FRAP) using enhanced green fluorescent protein-tagged histone H2B. The results indicated that the chromatin in growing oocytes was already highly loosened to a level comparable to that in early preimplantation embryos. To elucidate the mechanism underlying the loosened chromatin structure in oocytes, we focused on chromodomain helicase DNA binding protein 9 (Chd9), which is highly expressed in growing oocytes. The oocytes from Chd9 knockout mice (Chd9(-/-)) generated using the CRISPR/Cas9 system exhibited a less loosened chromatin structure than that of wild-type mice, suggesting that Chd9 is involved in the loosened chromatin structure in growing oocytes. These results suggest that a loosened chromatin structure, which is mediated by Chd9, is a prerequisite for the acquisition of totipotency after fertilization. (C) 2018 Elsevier Inc. All rights reserved.