Phylogenetic relationships among Streptococcus agalactiae isolated from piscine, dolphin, bovine and human sources: a dolphin and piscine lineage associated with a fish epidemic in Kuwait is also associated with human neonatal infections in Japan
Phylogenetic relationships among Streptococcus agalactiae isolated from piscine, dolphin, bovine and human sources: a dolphin and piscine lineage associated with a fish epidemic in Kuwait is also associated with human neonatal infections in Japan
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DOI：
10.1099/jmm.0.47815-0
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发表时间：
2008-11-01
影响因子：
3
通讯作者：
Takahashi, Shinji
中科院分区：
文献类型：
--
作者：
Evans, Joyce J.;Bohnsack, John F.;Takahashi, Shinji
无乳链球菌，通常称为 B 族链球菌 (GBS)，是许多动物物种感染性疾病的原因。本研究利用血清学和分子血清分型以及多位点序列分型 (MLST) 技术，检查了来自不同地理区域的鱼、海豚和人类 GBS 分离株以及牛 GBS 参考株的遗传相关性。源自科威特、巴西、以色列和美国的鱼类分离株为荚膜血清型 la，这是一种以前从未在从鱼类中分离出的 GBS 中报道过的血清型。鱼类分离株的序列分型产生了六种序列类型（ST-7、ST-257、ST-258、ST-259、ST-260 和 ST-261），后五种代表先前在无乳链球菌 MLST 数据库中未报道的等位基因名称和等位基因组合。来自科威特和其他地理区域的海豚和鱼类 GBS 分离株之间存在基因组多样性。来自巴西、以色列、洪都拉斯和美国的鱼类 GBS 分离株似乎代表了一个独特的菌株遗传群体，这些菌株在很大程度上与人类和牛 GBS 无关。科威特海豚和鱼类谱系（ST-7，la）也与日本的人类新生儿感染有关。鱼、人类和牛 GBS 的比较基因组学可以帮助阐明那些对宿主向性、独特致病性克隆的出现以及这些宿主是否充当彼此致病谱系的储存库很重要的基因。
Streptococcus agalactiae, commonly known as group B streptococcus (GBS), is a cause of infectious disease in numerous animal species. This study examined the genetic relatedness of piscine, dolphin and human GBS isolates and bovine GBS reference strains from different geographical regions using serological and molecular serotyping and multilocus sequence typing (MLST) techniques. Piscine isolates originating from Kuwait, Brazil, Israel and the USA were capsular serotype la, a serotype previously unreported in GBS isolated from fish. Sequence typing of piscine isolates produced six sequence types (ST-7, ST-257, ST-258, ST-259, ST-260 and ST-261), the latter five representing allelic designations and allelic combinations not previously reported in the S. agalactiae MLST database. Genomic diversity existed between dolphin and piscine GBS isolates from Kuwait and other geographical areas. Piscine GBS isolates from Brazil, Israel, Honduras and the USA appeared to represent a distinct genetic population of strains that were largely unrelated to human and bovine GBS. The Kuwait dolphin and piscine lineage (ST-7, la) was also associated with human neonatal infections in Japan. Comparative genomics of piscine, human and bovine GBS could help clarify those genes important for host tropism, the emergence of unique pathogenic clones and whether these hosts act as reservoirs of one another's pathogenic lineages.