Identification of miRNAs Affecting the Establishment of Brassica Alboglabra Seedling.
Identification of miRNAs Affecting the Establishment of Brassica Alboglabra Seedling.
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影响白菜幼苗建立的 miRNA 鉴定
DOI：
10.3389/fpls.2016.01760
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发表时间：
2016
影响因子：
5.6
通讯作者：
Lai Z
中科院分区：
文献类型：
--
作者：
Guo R;Deng Y;Huang Z;Chen X;XuHan X;Lai Z
microRNA（miRNAs）对植物发育包括种子形成、休眠和萌发以及幼苗建立是重要的。芸苔属蔬菜的成苗期不仅影响着优质苗的发育，而且也影响着芽苗菜的营养成分含量。以芥蓝（Brassica alboglabra）不同生长期幼苗为材料，构建了两个小分子RNA（sRNA）文库。我们综合分析了2天和9天龄幼苗中的miRNAs。在去除低质量读段和衔接子污染物后，平均生成11，722，490个干净读段。结果表明，2日龄和9日龄幼苗中分别有37.65%和26.69%的sRNA长度为24 nt。共鉴定了228个miRNA家族的254个成熟miRNA序列和343个新的miRNA序列。在这些miRNAs中，224个在两个分析文库之间差异表达。通过序列同源性鉴定的最丰富的miRNAs是miR 156、miR 167和miR 157，每个都具有超过100，000个测序读数。与2日龄幼苗相比，9日龄幼苗中miR 8154和miR 390的表达量分别上调和下调最多。对差异表达的影响生物过程的miRNA靶基因进行基因本体富集分析，发现大多数基因属于“转录调控”类别。此外，通过定量实时聚合酶链反应验证了一些miRNA和靶基因的表达模式。我们确定发育相关的miRNA（例如，bal-miR 156/157/159/166/167/172/396）、胁迫相关（bal-miR 408）和代谢相关（bal-miR 826）miRNAs在成苗期高表达。结合靶点SPL 9和AP 2的低水平，可以得出结论，miR 156-SPL 9和miR 172-AP模块在B过程中发挥关键作用。白花草幼苗建成期。
MicroRNAs (miRNAs) are important for plant development including seed formation, dormancy, and germination, as well as seedling establishment. The Brassica vegetable seedling establishment stage influences the development of high quality seedlings, but also affects the nutrient content of sprouts. Chinese kale (Brassica alboglabra) seedlings at different growth stages were used to construct two small-RNA (sRNA) libraries. We comprehensively analyzed the miRNAs in 2- and 9-day-old seedlings. An average of 11,722,490 clean reads were generated after removing low-quality reads and adapter contaminants. The results revealed that 37.65 and 26.69% of the sRNAs in 2- and 9-day-old seedlings, respectively, were 24 nt long. In total, 254 known mature miRNA sequences from 228 miRNA families and 343 novel miRNAs were identified. Of these miRNAs, 224 were differentially expressed between the two analyzed libraries. The most abundant miRNAs identified by sequence homology were miR156, miR167, and miR157, each with more than 100,000 sequenced reads. Compared with the expression levels in 2-day-old seedlings, MiR8154 and miR390 were the most up- and down-regulated miRNAs respectively in 9-day-old seedlings. Gene ontology enrichment analysis of the differentially expressed-miRNA target genes affecting biological processes revealed that most genes were in the “regulation of transcription” category. Additionally, the expression patterns of some miRNAs and target genes were validated by quantitative real-time polymerase chain reaction. We determined that development-associated miRNAs (e.g., bal-miR156/157/159/166/167/172/396), were highly-expressed during seedling-establishment stage, as were stress-related (bal-miR408) and metabolism-related (bal-miR826) miRNAs. Combined with the low level of targets SPL9 and AP2, it was concluded that miR156-SPL9 and miR172-AP modules play key roles during the B. alboglabra seedling establishment stage.
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影响因子：
7.2
作者：
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通讯作者：
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影响因子：
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通讯作者：
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