Construction of high-density genetic linkage map of Pyropia yezoensis (Bangiales, Rhodophyta) and identification of red color trait QTLs in the thalli
Construction of high-density genetic linkage map of Pyropia yezoensis (Bangiales, Rhodophyta) and identification of red color trait QTLs in the thalli
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DOI：
10.1007/s00343-020-0184-5
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发表时间：
2021-01
影响因子：
1.6
通讯作者：
Lu Wang;Kuipeng Xu;Xianghai Tang;Junhao Wang;Fanna Kong;Y. Mao
中科院分区：
文献类型：
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作者：
Lu Wang;Kuipeng Xu;Xianghai Tang;Junhao Wang;Fanna Kong;Y. Mao
条斑热带藻是一种重要的大型藻类，具有广泛的全球分布和重要的经济价值。颜色是Talli OFP的一个重要特征。在遗传育种中，它也是鉴定杂交的有效标记。本研究基于高通量单核苷酸多态(SNP)标记构建了一张高密度遗传连锁图谱，并用于分析叶状体红色性状的数量性状座位(QTL)。软体孢子经过减数分裂发育成有序的四分体，每个细胞都有单倍体表型，并能生长成单个个体。在此基础上，以F1单倍体群体为作图群体。该图谱包含531个SNP标记，平均长度为394.57 cM，平均距离为0.74 cM。遗传连锁图谱与物理图谱的共线分析表明，两个图谱的覆盖率为79.42%。此外，QTL定位在与菌体红色性状相关的染色体区域发现了6个QTL。单个QTL的表型方差解释(PVE)值在4.71%~63.11%之间。QTLqRed-1-1，PVE为63.11%。因此，这些数据可能为基因和QTL精细定位以及分子标记辅助育种提供平台。是对未来的敏感。
Pyropia yezoensisis an important macroalga because of its extensive global distribution and economic importance. Color is an important trait in the thalli ofP. yezoensis, it is also an effective marker to identify the hybridization in genetic breeding. In this study, a high-density genetic linkage map was constructed based on high-throughput single nucleotide polymorphism (SNP) markers, and used for analyzing the quantitative trait loci (QTLs) of red color trait in the thalli ofP. yezoensis.The conchospore undergoes meiosis to develop into an ordered tetrad, and each cell has a haploid phenotype and can grow into a single individual. Based on this theory, F1 haploid population was used as the mapping population. The map included 531 SNP markers, 394.57 cM long on average distance of 0.74 cM. Collinear analysis of the genetic linkage map and the physical map indicated that the coverage between the two maps was 79.42%. Furthermore, QTL mapping identified six QTLs for the chromosomal regions associated with the red color trait of the thalli. The value of phenotypic variance explained (PVE) by an individual QTL ranged from 4.71%–63.11%. And QTLqRed-1-1, with a PVE of 63.11%, was considered the major QTL. Thus, these data may provide a platform for gene and QTL fine mapping, and marker-assisted breeding inP. yezoensisin the future.