Isolation of a methane-oxidizing bacterium that bioremediates hexavalent chromium from a formerly industrialized Suburban River
Isolation of a methane-oxidizing bacterium that bioremediates hexavalent chromium from a formerly industrialized Suburban River
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DOI：
10.1111/lam.13330
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发表时间：
2020-06-18
影响因子：
2.4
通讯作者：
Smith, T. J.
中科院分区：
文献类型：
--
作者：
Challa, S.;Smith, T. J.
沉积物样本取自英格兰北部谢菲尔德郊区一条河流附近的沉积物，该河流由于之前的钢铁工业活动（17 世纪至 19 世纪）而遭受重金属污染。样品中含量最高的重金属为铅、铬、镍、砷和钴，最高浓度分别为412点80、25点232、25点196、8点123和7点66 mg kg(-1)。使用甲烷作为碳和能源建立富集培养物，结果分离出一株甲烷氧化菌，通过16S rRNA基因测序显示该菌株是甲基单胞菌koyamae菌株，并命名为SHU1.M。 koyamaeSHU1 从 10 ppm 的初始浓度中去除了六价铬，该六价铬受到代谢抑制剂叠氮化钠或甲烷单加氧酶抑制剂苯乙炔的抑制。据作者所知，这是对广泛环境分布的甲基单胞菌属菌株的首次描述，该菌株能够修复六价铬。
Sediment samples were taken from sediment adjacent to a suburban river in Sheffield in Northern England that had suffered heavy metal pollution due to previous activity of the steel industry (between the 17th and 19th centuries). The most abundant heavy metals found in the samples were lead, chromium, nickel, arsenic and cobalt, with maximum concentrations of 412 center dot 80, 25 center dot 232, 25 center dot 196, 8 center dot 123 and 7 center dot 66 mg kg(-1), respectively. Enrichment cultures were set up using methane as carbon and energy source, as a result of which a strain of methanotroph was isolated that was shown via 16S rRNA gene sequencing to be a strainMethylomonas koyamaeand given the designation SHU1.M. koyamaeSHU1 removed hexavalent chromium from an initial concentration of 10 ppm, which was inhibited by the metabolic inhibitor sodium azide or the methane monooxygenase inhibitor phenylacetylene. To the authors' knowledge, this is the first description of a strain of the widely environmentally distributed genusMethylomonasthat is capable of remediating hexavalent chromium.