Novel kingdom-level eukaryotic diversity in anoxic environments
Novel kingdom-level eukaryotic diversity in anoxic environments
复制标题
DOI：
10.1073/pnas.062169599
复制
发表时间：
2002-06-11
影响因子：
11.1
通讯作者：
Pace, NR
中科院分区：
文献类型：
--
作者：
Dawson, SC;Pace, NR
真核生物的分子进化研究依赖于稀疏的基因序列集合，这些基因序列不代表自然界中真核生物多样性的全部范围。特别是厌氧微生物，在系统发育研究中几乎没有代表性。这类生物是真核生物中最不为人所知的，可能也是最具遗传多样性的。为了给缺氧环境中真核生物的自然多样性提供新的视角，同时也为了发现新的序列用于进化研究，我们对三个缺氧沉积物进行了一个独立于培养的分子系统发育调查，包括淡水和海洋样品，鉴定了许多以前未被识别的真核生物，包括七个血统的代表，这些血统与王国级别的任何已知生物都没有特别的关系，该调查还鉴定了具有厌氧成员的已知生态重要真核生物群的新序列特征。系统发育分析与新的序列增强了我们对真核生物进化的多样性和模式的理解。
Molecular evolutionary studies of eukaryotes have relied on a sparse collection of gene sequences that do not represent the full range of eukaryotic diversity in nature. Anaerobic microbes, particularly, have had little representation in phylogenetic studies. Such organisms are the least known of eukaryotes and probably are the most phylogenetically diverse. To provide fresh perspective on the natural diversity of eukaryotes in anoxic environments and also to discover novel sequences for evolutionary studies, we conducted a cultivation-independent, molecular phylogenetic survey of three anoxic sediments, including both freshwater and marine samples, Many previously unrecognized eukaryotes were identified, including representatives of seven lineages that are not specifically related to any known organisms at the kingdom-level and branch below the eukaryotic "crown" radiation of animals, plants, fungi, stramenopiles, etc. The survey additionally identified new sequences characteristic of known ecologically important eukaryotic groups with anaerobic members. Phylogenetic analyses with the new sequences enhance our understanding of the diversity and pattern of eukaryotic evolution.