G protein Gαo is essential for vomeronasal function and aggressive behavior in mice
G protein Gαo is essential for vomeronasal function and aggressive behavior in mice
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DOI：
10.1073/pnas.1107770108
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发表时间：
2011-08-02
影响因子：
11.1
通讯作者：
Leinders-Zufall, Trese
中科院分区：
文献类型：
--
作者：
Chamero, Pablo;Katsoulidou, Vicky;Leinders-Zufall, Trese
啮齿动物犁鼻器官（VNO）调节着物种间和物种间的社会行为。我们使用基因靶向来检测由基因Gnao1编码的G蛋白G α 0在犁鼻功能中的作用。我们使用ccrep - loxp系统删除了表达嗅觉标记蛋白的细胞中的G α 0，其中包括VNO感觉上皮基底层的所有vomeronasal感觉神经元。通过电生理学和钙成像，我们发现条件缺失小鼠对特定分子信号（包括MHC1抗原、主要尿蛋白和外分泌腺分泌肽）表达V2R受体的颏鼻感觉神经元的感觉反应显著降低。G α o对于两种来自NADH脱氢酶1的n -甲酰化线粒体编码肽的颏鼻感应也是至关重要的。此外，我们还发现G α o是雌雄领地攻击和母性攻击在小鼠中表现出来的必要条件。最后，我们发现G α o依赖性母性攻击可以由主要的尿蛋白诱导。这些细胞和行为表型表明G α 0是主要的G蛋白α亚基，介导VNO感觉神经元对肽和蛋白质信息素的检测。
The rodent vomeronasal organ (VNO) mediates the regulation of species-specific and interspecies social behaviors. We have used gene targeting to examine the role of the G protein G alpha o, encoded by the gene Gnao1, in vomeronasal function. We used the Cre-loxP system to delete G alpha o in those cells that express olfactory marker protein, which includes all vomeronasal sensory neurons of the basal layer of the VNO sensory epithelium. Using electrophysiology and calcium imaging, we show that the conditional null mice exhibit strikingly reduced sensory responses in V2R receptor-expressing vomeronasal sensory neurons to specific molecular cues, including MHC1 antigens, major urinary proteins, and exocrine gland-secreting peptide. G alpha o is also vital for vomeronasal sensing of two N-formylated mitochondrially encoded peptides derived from NADH dehydrogenase 1. Furthermore, we show that G alpha o is an essential requirement for the display of male-male territorial aggression as well as maternal aggression in mice. Finally, we show that G alpha o-dependent maternal aggression can be induced by major urinary proteins. These cellular and behavioral phenotypes identify G alpha o as the primary G-protein alpha-subunit mediating the detection of peptide and protein pheromones by sensory neurons of the VNO.