The human placental proteome is affected by maternal smoking.
The human placental proteome is affected by maternal smoking.
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DOI：
10.1016/j.reprotox.2016.05.009
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Pasanen M
中科院分区：
文献类型：
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作者：
Huuskonen P;Amezaga MR;Bellingham M;Jones LH;Storvik M;Häkkinen M;Keski-Nisula L;Heinonen S;O'Shaughnessy PJ;Fowler PA;Pasanen M
研究了母亲吸烟对足月胎盘蛋白质组的影响。母亲吸烟显着影响18%的蛋白质点。母亲吸烟影响控制胎盘发育和功能的系统。观察到的胎盘变化可能导致出生体重降低。通过使用7个非吸烟者和7个吸烟者胎盘，研究了母亲吸烟对胎盘蛋白质组和类固醇代谢活动以及母体激素水平的有害影响。吸烟显著影响了18%的蛋白质点。受影响的功能网络是i）细胞形态、细胞组装和组织、细胞妥协（15次命中）和ii）DNA复制、重组和修复、能量产生、核酸代谢（6次命中）。吸烟显著上调SERPINA 1、EFHD 1和KRT 8等蛋白;下调SERPINB 2、FGA和HBB。虽然母体血浆类固醇没有显着改变，CYP 1A 1的催化活性增加，而CYP 19 A1活性降低吸烟。此外，吸烟诱导CYP 1A 1和CYP 4 B1的转录表达，而抑制HSD 17 B2、NF κ B和TGF β 1。观察到的吸烟诱导的胎盘蛋白质组和转录水平的广泛变化可能有助于新生儿和胎盘的出生体重降低。
The effects of maternal smoking on the term placental proteome was studied. Maternal smoking significantly affected 18% of protein spots. Maternal smoking affects systems controlling the development and function of placenta. The observed placental changes may contribute to the lowered birth weights. Detrimental effects of maternal smoking on the term placental proteome and steroid-metabolizing activities, and maternal hormone levels, were studied by using seven non-smoker and seven smoker placentae. Smoking significantly affected 18% of protein spots. The functional networks affected were i) cell morphology, cellular assembly and organization, cellular compromise (15 hits) and ii) DNA replication, recombination, and repair, energy production, nucleic acid metabolism (6 hits). Smoking significantly up-regulated such proteins as, SERPINA1, EFHD1 and KRT8; and down-regulated SERPINB2, FGA and HBB. Although maternal plasma steroids were not significantly altered, the catalytic activity of CYP1A1 was increased whereas CYP19A1 activity was reduced by smoking. Furthermore, transcript expression of CYP1A1 and CYP4B1 were induced while HSD17B2, NFKB and TGFB1 were repressed by smoking. The observed smoking induced wide-spread changes on placental proteome and transcript levels may contribute to the lowered birth weights of the new-born child and placenta.