CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes.
CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes.
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DOI：
10.1093/intimm/dxm083
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发表时间：
2007-09
影响因子：
4.4
通讯作者：
M. Tohyama;K. Sayama;H. Komatsuzawa;Y. Hanakawa;Y. Shirakata;X. Dai;Lujun Yang;S. Tokumaru;H. Nagai;S. Hirakawa;M. Sugai;K. Hashimoto
中科院分区：
文献类型：
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作者：
M. Tohyama;K. Sayama;H. Komatsuzawa;Y. Hanakawa;Y. Shirakata;X. Dai;Lujun Yang;S. Tokumaru;H. Nagai;S. Hirakawa;M. Sugai;K. Hashimoto
表皮不断地暴露在各种微生物病原体中，并在抵抗它们方面发挥着至关重要的作用。可溶性CXC趋化因子配体(CXCL)16是ELR-CXC趋化因子之一，通过吸引CXC趋化因子受体(CXCR)6表达的细胞，如活化的T细胞和NKT细胞，发挥天然免疫的调节作用。然而，非免疫细胞产生CXCL16的机制尚不清楚。我们发现，培养的角质形成细胞产生大量的CXCL16(每10(6)个细胞每24小时产生2-3 ng)。肿瘤坏死因子α、IL-1α、干扰素-γ、多肽聚糖和多聚肌苷多胞苷[Poly(I：C)]刺激促进CXCL16的产生。培养上清液中CXCL16的相对分子质量分别为14、28和50 kDa。免疫组织化学分析表明，正常人表皮表达CXCL16。鉴于几种趋化因子具有抗微生物活性，我们研究了CXCL16的抗菌活性。CXCL16的趋化素结构域在浓度为5微克/毫升(-1)时对金黄色葡萄球菌和大肠杆菌具有显著的抑菌活性。在碳酸盐存在的情况下，杀灭活性保持在生理盐分浓度。综上所述，CXCL16是一种新的表皮角质形成细胞天然免疫反应的介体。
The epidermis is constantly exposed to a variety of microbial pathogens and plays a vital role in resisting them. Soluble CXC chemokine ligand (CXCL) 16, which is one of the ELR- CXC chemokines, acts as a mediator of innate immunity by attracting CXC chemokine receptor (CXCR) 6-expressing cells, such as activated T cells and NKT cells. However, the production of CXCL16 by non-immune cells remains unclear. We found that cultured keratinocytes produced a significant amount of CXCL16 (2-3 ng per 10(6) cells per 24 h). Stimulation with tumor necrosis factor alpha, IL-1alpha, IFN-gamma, peptidoglycan and polyinosinic-polycytidylic acid [poly(I:C)] enhanced CXCL16 production. The forms of CXCL16 in the culture supernatants had molecular weights of 14, 28 and 50 kDa. Immunohistochemical analysis revealed that the normal human epidermis expressed CXCL16. As several chemokines have anti-microbial activities, we studied the anti-microbial activity of CXCL16. The chemokine domain of CXCL16 at concentrations >5 microg ml(-1) had significant anti-microbial activity against Staphylococcus aureus and Escherichia coli. Killing activity was retained at the physiological salt concentration in the presence of carbonate. In conclusion, CXCL16 is a novel mediator of the innate immune reactivities of epidermal keratinocytes.