Diminished microtubule-associated protein 2 (MAP2) immunoreactivity following cortical impact brain injury
Diminished microtubule-associated protein 2 (MAP2) immunoreactivity following cortical impact brain injury
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DOI：
10.1089/neu.1996.13.125
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发表时间：
1996-03-01
影响因子：
4.2
通讯作者：
Hayes, RL
中科院分区：
文献类型：
--
作者：
Posmantur, RM;Kampfl, A;Hayes, RL
本研究采用Western blotting和定性免疫组织化学方法分析了皮质性撞击性脑损伤（TBI）对大鼠皮质MAP2免疫反应性的影响。我们采用外侧皮质撞击损伤装置诱导严重的TBI，其伴有局灶性皮质挫伤和撞击部位神经元死亡，TBI后3小时，Western blotting检测到仅在损伤部位同侧皮质大量MAP2丢失。MAP2的光镜研究显示，在第3层和第5层锥体神经元的顶端树突中，MAP2免疫荧光的明显丧失，以及第1层精细树突的丧失。MAP2免疫标记的这些变化与苏木精和伊红染色标记的黑暗萎缩神经元的存在有关，但不仅限于此，这表明细胞即将死亡。在同侧皮质局灶性挫伤和坏死区域内外都发现了MAP2免疫荧光的改变，因此，大鼠创伤性脑损伤可以在挫伤区域内产生快速而显著的树突状病理，然而，在挫伤区域外MAP2标记的免疫组织化学变化表明，tbi诱导的树突状损伤可能不仅仅与急性细胞死亡有关。
This study employed Western blotting and qualitative immunohistochemistry to analyze the effects of cortical impact traumatic brain injury (TBI) on acute changes in MAP2 immunoreactivity in the rat cortex, We employed a lateral cortical impact injury device to induce severe TBI, which is associated with focal cortical contusion and neuronal death at the impact site, Three hours following TBI, Western blotting detected substantial MAP2 loss only in the cortex ipsilateral to the site of injury, Light microscopic studies of MAP2 revealed a prominent loss of MAP2 immunofluorescence in apical dendrites of pyramidal neurons within layers 3 and 5, as well as a loss of fine dendritic arborization within layer 1, These changes in MAP2 immunolabeling were associated with, but not exclusively restricted to, the presence of dark shrunken neurons labeled by hematoxylin and eosin staining, suggesting impending cell death, Alterations in MAP2 immunofluorescence were found both within and beyond areas of focal contusion and necrosis in the ipsilateral cortex, Thus, traumatic brain injury in rats can produce rapid and significant dendritic pathology within sites of contusion, However, immunohistochemical changes in MAP2 labeling outside of contused regions suggests that TBI-induced dendritic damage may not be exclusively associated with acute cell death.