Characterization of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neoplastic epithelial cells of human mammary gland.
Characterization of a glycosphingolipid antigen defined by the monoclonal antibody MBr1 expressed in normal and neoplastic epithelial cells of human mammary gland.
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DOI：
10.1016/s0021-9258(17)42669-x
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发表时间：
1984-12
期刊：
影响因子：
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通讯作者：
E. Bremer;S. Levery;Sandro Sonnino;Riccardo Ghidoni;Silvana Canevari;Reiji Kannagi;S. Hakomori
中科院分区：
文献类型：
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作者：
E. Bremer;S. Levery;Sandro Sonnino;Riccardo Ghidoni;Silvana Canevari;Reiji Kannagi;S. Hakomori
单克隆抗体定义的抗原，MBr1，被发现在正常人乳腺上皮细胞和人乳腺癌细胞中表达（msamadard， S., Tagliabue， E., Canevari， S., Fossati， G., and Colnaghi, m.i. (1983) Cancer Res. 43, 1295-1300）。该抗原已从乳腺癌细胞系MCF-7中分离出来，用作免疫原，并通过甲基化分析、核磁共振光谱、直接探针质谱和酶降解确定其结构，如下所述。Fuc α 1—2Gal β 1—3GalNAc β 1—3Gal α 1—4Gal β 1—4Glc β 1—1Cer抗体与focusylasialo-GM1 （IV2FucGg4）发生弱交叉反应，后者与该抗原具有相同的末端序列Fuc α 1—2Gal β 1—3GalNAc。然而，其他各种结构，包括乳系列H结构（Fuc α 1—2 Gal β 1—4/或3GlcNAc β 1—3Gal），与该抗体没有任何反应性。因此，该抗原代表了一种具有全球系列结构的血型H抗原，在人类畸形体癌中大量存在(Kannagi， R., Levery, s.b ., Ishigami， F., Hakomori， S., Shevinsky, l.h ., Knowles, b.b ., and Solter， D.（1983）。化学，258,8934-8942)，虽然它的存在必须限制在正常成人组织。
The antigen defined by a monoclonal antibody, MBr1, was found to be expressed in normal human mammary gland epithelia and human mammary carcinoma cells (Ménard, S., Tagliabue, E., Canevari, S., Fossati, G., and Colnaghi, M. I. (1983) Cancer Res. 43, 1295-1300). The antigen has been isolated from breast cancer cell line MCF-7, which was used as immunogen, and its structure was determined by methylation analysis, NMR spectroscopy, direct probe mass spectrometry, and enzymatic degradation as identified below. Fuc alpha 1—-2Gal beta 1—-3GalNAc beta 1—-3Gal alpha 1—-4Gal beta 1—-4Glc beta 1—-1Cer The antibody cross-reacted weakly with fucosylasialo-GM1 (IV2FucGg4), which shares the same terminal sequence, Fuc alpha 1—-2Gal beta 1—-3GalNAc, with this antigen. However, various other structures, including lacto-series H structure (Fuc alpha 1—-2 Gal beta 1—-4/or 3GlcNAc beta 1—-3Gal), did not show any reactivity with this antibody. Therefore, this antigen represents a blood group H antigen with a globo-series structure which is abundant in human teratocarcinoma (Kannagi, R., Levery, S. B., Ishigami, F., Hakomori, S., Shevinsky, L. H., Knowles, B. B., and Solter, D. (1983) J. Biol. Chem. 258, 8934-8942), although its presence must be limited in normal adult human tissue.