Antibodies to distal carboxyl terminal epitopes in the v-fms-coded glycoprotein do not cross-react with the c-fms gene product.
Antibodies to distal carboxyl terminal epitopes in the v-fms-coded glycoprotein do not cross-react with the c-fms gene product.
复制标题
针对 v-fms 编码糖蛋白中远端羧基末端表位的抗体不会与 c-fms 基因产物发生交叉反应。
DOI：
10.1016/0042-6822(86)90145-5
复制
发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Sherr,CJ
中科院分区：
文献类型：
--
作者：
Furman,WL;Rettenmier,CW;Chen,JH;Roussel,MF;Quinn,CO;Sherr,CJ
v-fmsoncogene 的产物是一种完整的跨膜糖蛋白，与巨噬细胞集落刺激因子 CSF-1 的细胞表面受体密切相关。将编码糖蛋白的胞外氨基末端结构域的主要部分、跨膜片段和整个羧基末端酪氨酸激酶结构域的v-fms基因片段分子克隆到诱导型原核表达质粒中。仅由 v-fms 编码的氨基酸组成的多肽产品在细菌中产生，并用于制备免疫试剂，沉淀转化细胞中表达的 v-fms 编码的糖蛋白。针对重组多肽的兔抗血清检测到了 c-fms 原癌基因产物的抗原决定簇，而针对这些相同抗原的七种小鼠单克隆抗体仅与 v-fms 特异性表位发生反应。对缺乏野生型产品的 37 个羧基末端氨基酸的突变体 v-fms 编码的糖蛋白进行的蛋白水解作图实验和研究表明，单克隆抗体对糖蛋白最羧基末端表位的反应性受到限制。 v-fms 和 c-fmsgene 产品在该区域肯定有显着差异。
The product of the v-fmsoncogene is an integral transmembrane glycoprotein that is closely related to the cell surface receptor for the macrophage colony stimulating factor, CSF-1. A fragment of the v-fmsgene encoding a major portion of the extracellular amino terminal domain, the membrane-spanning segment, and the entire carboxyl terminal tyrosine kinase domain of the glycoprotein was molecularly cloned into an inducible prokaryotic expression plasmid. Polypeptide products consisting only of v-fms-coded amino acids were produced in bacteria and were used to prepare immune reagents that precipitated the v-fms-coded glycoproteins expressed in transformed cells. Whereas rabbit antisera to recombinant polypeptides detected antigenic determinants of the c-fmsproto-oncogene product, seven mouse monoclonal antibodies to these same antigens reacted only with v-fms-specific epitopes. Proteolytic mapping experiments and studies with a mutant v-fms-coded glycoprotein lacking the 37 carboxyl terminal amino acids of the wild-type product showed that the monoclonal antibodies were restricted in their reactivity to epitopes at the extreme carboxyl terminus of the glycoprotein. The v-fmsand c-fmsgene products must differ significantly in this region.