The onset of germ cell migration in the mouse embryo
The onset of germ cell migration in the mouse embryo
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DOI：
10.1016/s0925-4773(99)00271-3
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发表时间：
2000-03-01
影响因子：
2.6
通讯作者：
Wylie, C
中科院分区：
文献类型：
--
作者：
Anderson, R;Copeland, TK;Wylie, C
小鼠原始生殖细胞（PGCs）在胚胎第6.5天（E6.5）和E7.5之间被指定，当它们在发育中的尿囊中被视为碱性磷酸酶阳性（AP+）细胞群时。到E8.5，它们嵌入后肠上皮中。以前的实验表明PGCs的起源不同，目前还不清楚它们是如何到达肠道上皮的。我们使用了在截短的Oct 4启动子下表达GFP的转基因小鼠来观察活的PGC。我们发现GFP+/AP+细胞在后端的原条作为一个分散的细胞群体积极迁移到尿囊，并直接进入相邻的胚胎内胚层。时间推移分析显示，这些细胞是积极迁移的时候，他们退出的原始条纹。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Mouse primordial germ cells (PGCs) are specified between embryonic day 6.5 (E6.5) and E7.5, when they have been visualized as an alkaline phosphatase-positive (AP+) cell population in the developing allantois. By E8.5, they are embedded in the hind-gut epithelium. Previous experiments have suggested different sites for PGCs' origin, and it is unclear how they reach the gut epithelium. We have used transgenic mice expressing GFP under a truncated Oct4 promoter to visualize living PGCs. We find GFP+/AP+ cells in the posterior end of the primitive streak as a dispersed population of cells actively migrating into the allantois, and directly into the adjacent embryonic endoderm. Time-lapse analysis shows these cells to be actively migratory from the time they exit the primitive streak. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.