EFFECTS OF ALUMINUM SALTS ON CULTURED NEUROBLASTOMA CELLS
EFFECTS OF ALUMINUM SALTS ON CULTURED NEUROBLASTOMA CELLS
复制标题
铝盐对培养的神经母细胞瘤细胞的影响
DOI：
10.1111/j.1471-4159.1974.tb04290.x
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发表时间：
1974
影响因子：
4.7
通讯作者：
Elliot M. Levine
中科院分区：
文献类型：
--
作者：
Carol A. Miller;Elliot M. Levine
几年前，在注射铝盐的兔子的中枢神经系统中，实验性地诱导了神经原纤维缠结。在目前的研究中，神经母细胞瘤细胞能够在单层培养中进行形态和生化分化，并暴露于含有磷酸铝的培养基中。连续暴露于铝盐 6 天后，这种处理导致 100 Å 神经丝的大量积累。虽然处理细胞中的生长速率和放射性胸苷的掺入与对照组相似，但总细胞蛋白和放射性亮氨酸的掺入显着增加。矛盾的是，当铝处理的培养物的蛋白质含量达到最大时，这些培养物每个细胞所含的核糖体 RNA 比对照培养物少约 20%。此外，一种重要的神经元蛋白（即乙酰胆碱酯酶）的活性在处理过的培养物中被抑制至低于对照值的水平。经处理的神经母细胞瘤培养物和注射铝盐的动物之间在时间和形态上的相似性表明，在细胞培养物中观察到的大分子合成变化与体内研究相关。
Experimental induction of neurofibrillary tangles was demonstrated several years ago in the central nervous system of rabbits injected with aluminum salts. In the current studies, neuroblastoma cells, capable of morphologic and biochemical differentiation in monolayer culture, have been exposed to medium containing aluminum phosphate; such treatment resulted in an abundant accumulation of 100 Å neurofilaments after 6 days of continous exposure to the aluminum salt. While growth rates and incorporation of radioactive thymidine in treated cells remained similar to controls, total cellular protein, and incorporation of radioactive leucine were significantly increased. Paradoxically, when the protein content of aluminum‐treated cultures was maximal, these cultures contained about 20 per cent less ribosomal RNA per cell than in control cultures. In addition, activity of an important neuronal protein, i.e. acetylcholinesterase, was depressed in treated cultures to a level below control values. Both temporal and morphologic similarities between treated neuroblastoma cultures and animals injected with aluminum salts suggest that the observed changes in macromolecular synthesis in cell culture are relevant to in vivo studies.