Ultrastructural localization of antibody in differentiating plasma cells.
Ultrastructural localization of antibody in differentiating plasma cells.
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DOI：
10.1084/jem.127.1.109
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
Bouteille M
中科院分区：
文献类型：
--
作者：
Leduc EH;Avrameas S;Bouteille M
通过电子显微镜将抗体定位于超免疫兔脾脏的分化和成熟浆细胞内。辣根过氧化物酶用作抗原。在戊二醛固定的组织中，通过将过氧化物酶抗体与其抗原偶联，然后通过细胞化学方法显示过氧化物酶活性的位点，从而显示细胞内的过氧化物酶抗体。抗体首先出现在成血细胞的核周空间，在那里它通过分化为未成熟的浆细胞而持续存在，但在成熟的浆细胞中它从该位点消失。随着ergastoplasm的发育，抗体在许多但不是所有的池中积累。在浆细胞分化的所有阶段，抗体存在于高尔基体的板层部分。成熟的浆细胞表现出两种类型的抗体分布，一种是浓缩成大的球形脑池内颗粒，另一种是溢流到细胞质的所有部分。
Antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. Horseradish peroxidase was used as antigen. Intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. Antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but it disappears from this site in mature plasma cells. Concomitant with the development of the ergastoplasm, antibody accumulates in many but not all of its cisternae. Antibody is present in the lamellar portion of the Golgi apparatus in all phases of plasmacytic differentiation. Mature plasma cells exhibit two types of antibody distribution, a concentration into large spherical intracisternal granules or an overflowing into all parts of the cytoplasm.