Expression of pancreatitis associated proteins in urothelium and urinary afferent neurons following cyclophosphamide induced cystitis
Expression of pancreatitis associated proteins in urothelium and urinary afferent neurons following cyclophosphamide induced cystitis
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DOI：
10.1016/j.juro.2007.11.034
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发表时间：
2008-04-01
影响因子：
6.6
通讯作者：
Kiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Takahara, Yuki;Suzuki, Akinobu;Kiyama, Hiroshi
目的：应用免疫组织化学和逆转录聚合酶链式反应检测胰腺炎相关蛋白/再生基因家族成员在大鼠膀胱和背根神经节初级传入神经元中的表达。材料和方法：应用免疫组织化学和逆转录聚合酶链式反应技术，检测慢性膀胱炎动物模型注射Sigma(R)后4h、48h和10d后，胰腺炎相关蛋白-I和胰腺炎相关蛋白-III在大鼠膀胱和背根神经节中的表达。表达胰腺炎相关蛋白-I的细胞在背根神经节中可见，但在膀胱中未见。在正常背根神经节中，胰腺炎相关蛋白-I在一小部分小直径神经元(2.4%)中表达，这些神经元也呈异凝素-B4阳性。然而，在膀胱炎发生后10天，胰腺炎相关蛋白-I阳性神经元的数量增加(7.6%)，并在稍大的神经元群中进一步观察到胰腺炎相关蛋白-I免疫反应，以及酪氨酸激酶A阳性的小神经元。结论：目前的结果表明，胰腺炎相关蛋白-III与膀胱炎有关，它们参与了膀胱炎的异常感觉。
Purpose: We examined the expression profile of the members of the pancreatitis associated proteins/regenerating gene family in the bladder and in the primary afferent neurons of dorsal root ganglia using an animal model of cystitis.Materials and Methods: We examined the expression of pancreatitis-associated protein-I and pancreatitis-associated protein-III in the bladder and the dorsal root ganglia of female rats 4 hours, 48 hours or 10 days after cyclophosphamide (Sigma (R)) injection using immunohistochemistry and reverse transcriptase-polymerase chain reaction.Results: No pancreatitis-associated protein-III immunoreactivity was identified in control bladders but prominent expression was observed in the urothelium of animals with chronic cystitis. Cells expressing pancreatitis-associated protein-I were seen in the dorsal root ganglia but not in the bladder. In normal dorsal root ganglia pancreatitis-associated protein-I was expressed in a minor population of small diameter neurons (2.4%) that were also positive for isolectin-B4. However, by 10 days following the onset of cystitis the number of pancreatitis-associated protein-I positive neurons was increased (7.6%) and pancreatitis-associated protein-I immunoreactivity was further observed in a slightly larger group of neurons, and tyrosine kinase A positive small neurons.Conclusions: The current results suggest that pancreatitis-associated protein-III is associated with bladder inflammation and they implicate pancreatitis-associated protein-I in the abnormal sensation in cystitis.