Gene expression profiling of chicken cecal tonsils and ileum following oral exposure to soluble and PLGA-encapsulated CpG ODN, and lysate of Campylobacter jejuni
Gene expression profiling of chicken cecal tonsils and ileum following oral exposure to soluble and PLGA-encapsulated CpG ODN, and lysate of Campylobacter jejuni
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DOI：
10.1016/j.vetmic.2017.11.010
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发表时间：
2017-12-01
影响因子：
3.3
通讯作者：
Sharif, Shayan
中科院分区：
文献类型：
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作者：
Taha-Abdelaziz, Khaled;Alkie, Tamiru Negash;Sharif, Shayan
空肠弯曲杆菌（C. jejuni）是导致人类食源性疾病的主要细菌。受污染的鸡肉是人类感染的重要来源。鸡在临床上不受定植的影响，自然感染后的免疫反应对肠道细菌负荷的影响有限。肠道免疫反应的诱导可能导致鸡与弯曲杆菌共生关系的破裂。我们最近的研究表明，可溶性和聚D， l -乳酸-羟基乙酸（PLGA）包封的CpG寡脱氧核苷酸（ODN）以及空肠假梭菌裂解物可有效减轻鸡的肠负荷；然而，这种保护背后的机制尚未确定。本研究旨在探讨这些处理所产生的宿主反应机制。将可溶性CpG ODN、plga包封CpG ODN或空肠C. lysate分别口服给鸡，采用定量RT-PCR技术检测盲肠扁桃体和回肠细胞因子和抗菌肽的表达。口服可溶性CpG ODN可上调干扰素(IFN)- γ、白细胞介素(IL)-1 β CXCLi2、转化生长因子(TGF)- β 4/1、IL-10和IL-13的表达，而plga包封CpG ODN可上调IL-1 β、CXCLi2、TGF- β 4/1、IL-13、禽d防御素（AvBD） 1、AvBD2和cathelicidin 3 （cathelicidin 3）的表达。空肠C.裂解物上调ifn - γ、IL-1 β、tgf - β 4/1、IL-13、AvBD1和CATHL-3的表达。综上所述，诱导肠道微环境中细胞因子和抗菌肽的表达可能是减少肉鸡空肠梭菌定植的一种手段，是减少人类弯曲杆菌病发病率的关键步骤。
Campylobacter jejuni (C. jejuni) is a leading bacterial cause of food-borne illness in humans. Contaminated chicken meat is an important source of infection for humans. Chickens are not clinically affected by colonization, and immune responses following natural infection have limited effects on bacterial-load in the gut. Induction of intestinal immune responses may possibly lead to a breakdown of the commensal relationship of chickens with Campylobacter. We have recently shown that soluble and poly D, L-lactic-co-glycolic acid (PLGA)-encapsulated CpG oligodeoxynucleotide (ODN) as well as C. jejuni lysate, are effective in reducing the intestinal burden of C. jejuni in chickens; however, the mechanisms behind this protection have yet to be determined. The present study was undertaken to investigate the mechanisms of host responses conferred by these treatments. Chickens were treated orally with soluble CpG ODN, or PLGA-encapsulated CpG ODN, or C. jejuni lysate, and expression of cytokines and antimicrobial peptides was evaluated in cecal tonsils and ileum using quantitative RT-PCR. Oral administration of soluble CpG ODN upregulated the expression of interferon (IFN)-gamma, interleukin (IL)-1 beta CXCLi2, transforming growth factor (TGF)-beta 4/1, IL-10 and IL-13, while treatment with PLGA-encapsulated CpG ODN upregulated the expression of IL-1 beta, CXCLi2, TGF-beta 4/1, IL-13, avian D-defensin (AvBD) 1, AvBD2 and cathelicidin 3 (CATHL-3). C. jejuni lysate upregulated the expression of IFN-gamma, IL-1 beta, TGF-beta 4/1, IL-13, AvBD1, and CATHL-3. In conclusion, induction of cytokine and antimicrobial peptides expression in intestinal micro environments may provide a means of reducing C. jejuni colonization in broiler chickens, a key step in reducing the incidence of campylobacteriosis in humans.