Insect Growth Inhibitory Activity of Clerodane Diterpenoids Isolated from Clerodendron infortunatum L. on the Cotton Bollworm, Helicoverpa armigera (Hubner)
Insect Growth Inhibitory Activity of Clerodane Diterpenoids Isolated from Clerodendron infortunatum L. on the Cotton Bollworm, Helicoverpa armigera (Hubner)
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DOI：
10.1007/s40009-012-0077-z
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发表时间：
2012-10-01
影响因子：
1.1
通讯作者：
Walia, Suresh
中科院分区：
文献类型：
--
作者：
Abbaszadeh, Gholamreza;Srivastava, Chitra;Walia, Suresh
从药用植物棘枝中分离得到3个克罗丹二萜类化合物，采用外用法和日粮混合法测定其对棉蚜的生长抑制活性，并与印楝素技术含量（20%）进行比较。采用幼虫死亡率、蛹死亡率、幼虫-蛹中间产物、异常蛹、蛹-成虫中间产物、幼虫和蛹减重、幼虫和蛹持续时间等参数评价其生长抑制效果。根据生长抑制指数（GI(50)）对本研究的生长抑制活性进行比较。通过外用观察，与GI（50）值为15 ppm的印楝素相比，克罗罗丁(1)、15-甲氧基-14,15-二氢克罗罗丁(2)和15-羟基-14,15-二氢克罗丁(3)的GI（50）值分别为13、21和11 ppm。通过日粮掺入法，印楝素即使在最低浓度（10 ppm）下也能完全抑制生长，纯化合物最低浓度下也能抑制50%以上的生长。结果表明，金盏花可作为开发植物性生物农药的重要植物。讨论了分离化合物对昆虫生长抑制各参数的影响。
Three clerodane diterpenoids were isolated from the medicinal plant, Clerodendron infortunatum and their growth inhibitory activity was evaluated against Helicoverpa armigera through topical as well as diet incorporation method and the results compared with azadirachtin technical (20 %). Different parameters were used to assess the growth inhibitory effects, such as larval mortality, pupal mortality, larval-pupal intermediates, abnormal pupae, pupal-adult intermediates, larval and pupal weight reduction and larval and pupal durations. The growth inhibition activity in the present study was compared based on growth inhibition index (GI(50)). It was observed that through topical application clerodin (1), 15-methoxy-14,15-dihydroclerodin (2) and 15-hydroxy-14,15-dihydroclerodin (3) showed 13, 21 and 11 ppm GI(50) values, respectively, compared to azadirachtin with GI(50) value of 15 ppm. Through diet incorporation method, azadirachtin showed complete growth inhibition even at the lowest concentration (10 ppm) and more than 50 % growth inhibition was recorded at the lowest concentration of the pure compounds. Results showed that C. infortunatum can be considered as an important plant to develop botanical biopesticides. Effect of isolated compounds on various parameters of insect growth inhibition is discussed.