Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections.
Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections.
复制标题
当用于喉部注射时，针头选择对肌肉衍生细胞存活的影响。
DOI：
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Halum S
中科院分区：
文献类型：
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作者：
Awonusi O;Harbin ZJ;Brookes S;Zhang L;Kaefer S;Morrison RA;Newman S;Voytik-Harbin S;Halum S
描述不同的注射器针头和递送载体如何影响喉注射时自体肌源性细胞（AMDC）的活力。在本研究中，收获成年猪肌肉组织并用于产生AMDC群体。在控制细胞浓度（1 × 107个细胞/ml）的同时，将包括肌肉祖细胞（MPC）或运动终板表达细胞（MEE）的AMDC悬浮在磷酸盐缓冲盐水或可聚合（原位支架形成）I型寡聚胶原溶液中。然后使用注射泵以相同的速率（2 ml/min）通过不同长度的23号和27号针注射细胞悬浮液。注射后立即以及注射后24小时和48小时测量细胞活力，然后与注射前的基线细胞活力进行比较。注射后细胞的活力不受针头长度或针头规格的影响，但受递送媒介物的显著影响。总的来说，使用胶原蛋白作为递送载体注射细胞保持了最高的细胞活力。针头规格、针头长度和递送媒介物是可影响注射细胞群的活力的重要因素。当用于喉部应用时，应考虑并调整这些因素以改善可注射MDC治疗结果。
To describe how differing injector needles and delivery vehicles impact Autologous Muscle-Derived Cell (AMDC) viability when used for laryngeal injection. In this study, adult porcine muscle tissue was harvested and used to create AMDC populations. While controlling cell concentration (1 × 107 cells/ml), AMDCs including Muscle Progenitor Cells (MPCs) or Motor Endplate Expressing Cells (MEEs) were suspended in either phosphate-buffered saline or polymerizable (in-situ scaffold forming) type I oligomeric collagen solution. Cell suspensions were then injected through 23- and 27-gauge needles of different lengths at the same rate (2 ml/min) using a syringe pump. Cell viability was measured immediately after injection and 24- and 48-hours post-injection, and then compared to baseline cell viability prior to injection. The viability of cells post-injection was not impacted by needle length or needle gauge but was significantly impacted by the delivery vehicle. Overall, injection of cells using collagen as a delivery vehicle maintained the highest cell viability. Needle gauge, needle length, and delivery vehicle are important factors that can affect the viability of injected cell populations. These factors should be considered and adapted to improve injectable MDC therapy outcomes when used for laryngeal applications.