The kinesin Klp2 mediates polarization of interphase microtubules in fission yeast
The kinesin Klp2 mediates polarization of interphase microtubules in fission yeast
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DOI：
10.1126/science.1113465
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发表时间：
2005-07-08
期刊：
影响因子：
56.9
通讯作者：
Nurse, P
中科院分区：
文献类型：
--
作者：
Carazo-Salas, RE;Antony, C;Nurse, P
裂殖酵母（粟酒裂殖酵母）细胞以一种依赖于其间期微管极化分布的方式纵向生长。我们发现这种分布需要微管沿着预先存在的微管向细胞中心滑动。这种滑动是由负端指向的驱动蛋白马达Klp2介导的，它帮助微管正确组织，使正端主要朝向细胞两端，负端朝向细胞中心。因此，裂殖酵母中的间期微管需要马达活动来实现其正确的组织。
Fission yeast (Schizosaccharomyces pombe) cells grow longitudinally in a manner dependent on a polarized distribution of their interphase microtubules. We found that this distribution required sliding of microtubules toward the cell center along preexisting microtubules. This sliding was mediated by the minus end-directed kinesin motor Klp2, which helped microtubules to become properly organized with plus ends predominantly oriented toward the cell ends and minus ends toward the cell center. Thus, interphase microtubules in the fission yeast require motor activities for their proper organization.