Chrysanthemum CmNAR2 interacts with CmNRT2 in the control of nitrate uptake.
Chrysanthemum CmNAR2 interacts with CmNRT2 in the control of nitrate uptake.
复制标题
菊花 CmNAR2 与 CmNRT2 相互作用控制硝酸盐吸收
DOI：
10.1038/srep05833
复制
发表时间：
2014-07-25
影响因子：
4.6
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Gu C;Zhang X;Jiang J;Guan Z;Zhao S;Fang W;Liao Y;Chen S;Chen F
硝酸盐转运蛋白是植物生长发育的重要组成部分。杭白菊是重要的观赏植物，为维持其经济产量，需要充足的氮肥供应。在这项研究中，全长cDNA的硝酸盐转运蛋白基因CmNRT 2和CmNAR 2的分离。CmNRT 2的转录积累是由硝酸盐和铵离子诱导的，但后者下调CmNAR 2的转录积累。CmNRT 2可能定位于质膜，而CmNAR 2则分布于整个细胞。通过体外和体内测定显示CmNAR 2与CmNRT 2相互作用。异源表达CmNRT 2的拟南芥植物表现出增加的硝酸盐流入速率，而表达CmNAR 2的植物中该性状未改变。双转化体（CmNRT 2加CmNAR 2）表现出增强的硝酸盐流入根的速率。我们的数据表明，CmNAR2与CmNRT2的相互作用有助于硝酸盐的吸收。
Nitrate transporters are an important component of plant growth and development. Chrysanthemum morifolium is an important ornamental species, for which a sufficient supply of nitrogenous fertilizer is required to maintain economic yields. In this study, the full-length cDNA of the nitrate transporter genes CmNRT2 and CmNAR2 were isolated. CmNRT2 transcript accumulation was inducible by both nitrate and ammonium, but the latter ion down-regulated the transcript accumulation of CmNAR2. CmNRT2 might be a plasma membrane localized protein, while CmNAR2 was distributed throughout the cell. CmNAR2 was shown to interact with CmNRT2 by in vitro and in vivo assays. Arabidopsis thaliana plants heterologously expressing CmNRT2 showed an increased rate of nitrate influx, while this trait was unaltered in plants expressing CmNAR2. Double transformants (CmNRT2 plus CmNAR2) exhibited an enhanced rate of nitrate influx into the root. Our data indicated that the interaction of CmNAR2 with CmNRT2 contributed to the uptake of nitrate.