Development of liquid chromatographic methods for determination of quinocetone and its main metabolites in edible tissues of swine and chicken.
Development of liquid chromatographic methods for determination of quinocetone and its main metabolites in edible tissues of swine and chicken.
复制标题
DOI：
10.1093/jaoac/88.2.472
复制
发表时间：
2005-03
影响因子：
1.6
通讯作者：
Lingli Huang;A. Xiao;S. Fan;Ju-yi Yin;Pin Chen;Dengcai Liu;Y. Qiu;Yulian Wang;Zonghui Yuan
中科院分区：
文献类型：
--
作者：
Lingli Huang;A. Xiao;S. Fan;Ju-yi Yin;Pin Chen;Dengcai Liu;Y. Qiu;Yulian Wang;Zonghui Yuan
喹烯酮（Quinocetone，QCT）是一种新型的喹酮类抗菌促生长剂，能有效地促进食用动物的生长和饲料效率，且安全性优于喹乙醇和卡巴肼。为明确其在动物体内的代谢和残留水平，建立了高效液相色谱-紫外-可见检测法测定猪和鸡肌肉、肝脏、肾脏和脂肪中QCT及其主要代谢产物脱氧喹烯酮（DQCT）和3-甲基喹喔啉-2-羧酸（MQCA）的方法。对于样品预处理，用乙酸乙酯提取QCT和DQCT，并用异辛烷纯化;在组织碱水解后，用乙酸乙酯和柠檬酸缓冲液（pH 6.0）提取MQCA，并在阳离子交换柱（AG MP-50树脂）上纯化提取物。QCT和DQCT的检测波长分别为312和320 nm，MQCA的检测波长为320 nm。在各种组织中观察到的3种化合物的检测限为0.025微克/克。该方法在0.01-0.64 μ g/mL浓度范围内呈线性，在0.05、0.10和0.20 μ g/g水平下，平均回收率约为71-86%，相对标准偏差约为4-12%。该方法选择性强，可用于动物组织中QCT及其主要代谢物的检测，为QCT在食用动物体内的药代动力学和残留研究提供了新的方法。
Quinocetone (QCT), a new antimicrobial growth promotant of quinoxalines, can effectively improve the growth and feed efficiency of food animals with more safety than is provided by olaquindox and carbadox. To clarify its metabolism and residue levels in animals, a liquid chromatographic method with UV-Vis detection was developed for the determination of QCT and its main metabolites, desoxyquinocetone (DQCT) and 3-methylquinoxaline-2-carboxylic acid (MQCA), in muscle, liver, kidney, and fat of swine and chicken. For sample pretreatment, QCT and DQCT were extracted with ethyl acetate and purified with iso-octane; after alkaline hydrolysis of the tissue, MQCA was extracted with ethyl acetate and citric acid buffer (pH 6.0), and the extract was purified over a cation-exchange column (AG MP-50 resin). Detection was at 312 and 320 nm for QCT and DQCT, respectively, and at 320 nm for MQCA. The observed limit of detection for the 3 compounds was 0.025 microg/g in various tissues. The methods were linear over the concentrations range of 0.01-0.64 microg/mL with mean recoveries of approximately 71-86% and relative standard deviations of about 4-12% at the levels of 0.05, 0.10, and 0.20 microg/g. The method is highly selective and can be applied to the determination of QCT and its main metabolites in animal tissues, which would accelerate the pharmacokinetic and residue study of QCT in food animals.