Stress selectively activates the vasopressin-containing subset of corticotropin-releasing hormone neurons.
Stress selectively activates the vasopressin-containing subset of corticotropin-releasing hormone neurons.
复制标题
压力选择性地激活促肾上腺皮质激素释放激素神经元中含有加压素的子集。
作者：
M. Whitnall
用功能性超微结构测定法测定了促肾上腺皮质激素释放激素（CRH）神经分泌细胞对短期应激的反应。消耗的神经分泌囊泡从轴突sediment在外部区的大鼠正中隆起被用来作为衡量的功能活动。一个小时的固定或5小时的胰岛素诱导的低血糖引起显着的消耗囊泡从加压素（VP）含有CRH轴突，但没有影响的VP缺乏亚群的CRH轴突。注射秋水仙碱（100微克）到侧脑室也导致在5小时的过程中选择性耗尽的囊泡从VP-含有亚群，没有耗尽从VP-缺陷的轴突。然而，注射100微克秋水仙碱后24小时，VP-含有和VP-缺乏轴突的神经分泌囊泡严重耗尽。这些数据首次表明，CRH神经分泌系统包含功能不同的组件，和VP分泌组件可能专门介导的促肾上腺皮质激素对短期压力的反应。
A functional ultrastructural assay was used to determine the response of corticotropin releasing hormone (CRH) neurosecretory cells to short-term stress. Depletion of neurosecretory vesicles from axonal swellings in the external zone of the rat median eminence was used as a measure of functional activity. One hour of immobilization or 5 h of insulin-induced hypoglycemia caused marked depletion of vesicles from the vasopressin (VP)-containing CRH axons, but had no effect on the VP-deficient subpopulation of CRH axons. Injection of colchicine (100 micrograms) into the lateral ventricle also resulted in selective depletion of vesicles from the VP-containing subpopulation over the course of 5 h, with no depletion from the VP-deficient axons. By 24 h after injection of 100 micrograms colchicine, however, both the VP-containing and the VP-deficient axons were severely depleted of neurosecretory vesicles. These data demonstrate for the first time that the CRH neurosecretory system contains functionally distinct components, and that the VP secreting component may specifically mediate the ACTH response to short-term stress.