Electrochemiluminescent metallopolymer coatings: Combined light and current detection in flow injection analysis
Electrochemiluminescent metallopolymer coatings: Combined light and current detection in flow injection analysis
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DOI：
10.1021/ac000605d
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发表时间：
2000-11-15
影响因子：
7.4
通讯作者：
Hogan, CF
中科院分区：
文献类型：
--
作者：
Forster, RA;Hogan, CF
报道了金属聚合物[Ru(bpy)(2)PVP10](2+)薄膜在流动注射分析系统中电化学发光(ECL)检测草酸盐的应用，其中bpy是2,2'-联吡啶，PVP是聚(4-乙烯基吡啶)。 ECL 试剂的固定化意味着它可以原位再生，无需不断地将其输送到反应区。电化学：生成的 Ru3+ 与分析物反应形成激发态 [Ru2+]*，在 610 nm 处发光。该反应在低pH值下是最佳的，此时该层会膨胀，并且通过该层的均匀电荷传输更容易。：与传统方法不同，我们同时监测修饰电极的电流和发光响应。两个信号的精度为：相似至 2% (n = 10)。然而，ECL 响应具有较大的动态范围，从低微摩尔范围延伸到高毫摩尔范围，并且检测下限较低，类似于注射 0.2 μM 或 4 pmol 的草酸盐。 ECL 方法对草酸盐表现出优异的选择性，对多种潜在干扰物（包括氧、胺、硫酸铁、硝酸铵、尿素和葡萄糖）具有出色的选择性。抗坏血酸是最显着的 ECL 干扰。然而，在 1 mM 抗坏血酸溶液中观察到的信号仍然存在。注射相似浓度的草酸盐时观察到的反应仅为 2.6%。
The application of thin films of the metallopolymer [Ru(bpy)(2)PVP10](2+) for the electrochemiluminescent (ECL) detection of oxalate in a flow injection analysis system is reported, where bpy is 2,2'-bipyridyl and PVP is poly(4-vinylpyridine). Immobilization of the ECL reagent means that it Can Be regenerated in situ, eliminating the need to constantly: deliver it to the reaction zone. Electrochemically:generated Ru3+ reacts with the analyte to form the excited-state [Ru2+]*, which luminesces at 610 nm. The reaction is optimal at low pH, where the layer is swollen and homogeneous charge transport through the layer is more facile.: Unlike traditional approaches, we simultaneously monitor both the amperometric and luminescent response of the modified electrode. The precision of both signals is:similar at similar to2% (n = 10). However, the ECL response has a larger dynamic range extending from the low-micromolar to higmillimolar range and a lower limit of detection, similar to0.2 muM or 4 pmol of oxalate injected. The ECL approach displays excellent selectivity for oxalate over:a wide range of potential interferences including oxygen, amines, iron sulfate, ammonium nitrate, urea, and glucose. Ascorbic acid represents the most significant ECL interference. However, the signal observed for a 1 mM solution of ascorbic acid is still. only 2.6% of the response Observed for the injection of a similar concentration of oxalate.