CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR A HUMAN HOMOLOG OF EUBACTERIAL ATP-DEPENDENT LON PROTEASES
CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR A HUMAN HOMOLOG OF EUBACTERIAL ATP-DEPENDENT LON PROTEASES
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DOI：
10.1016/0014-5793(94)80166-5
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发表时间：
1994-02-28
期刊：
影响因子：
3.5
通讯作者：
GORBALENYA, AE
中科院分区：
文献类型：
--
作者：
AMERIK, AY;PETUKHOVA, GV;GORBALENYA, AE
从人脑poly（A）（+）RNA制备的cDNA文库中分离含有推定的Lon蛋白酶（LonHS）的mRNA的重叠cDNA克隆。确定的核苷酸序列包含一个2814-bp的开放阅读框架，具有两个潜在的起始密码子（位置62-64和338-340）。LonHS mRNA的5 '端337个核苷酸片段高度富集G和C核苷酸，并且可以指导LonHS N端结构域的合成。更可能的是，该区域以帽非依赖性方式促进从第二AUG密码子开始的蛋白质合成。起始于第二个AUG密码子的氨基酸序列包括845个残基，其中超过30%与真细菌Lon蛋白酶的残基相同。NTP结合模式的“A”和“B”基序的残基以及可能的催化丝氨酸残基在LonHS中是保守的。北方印迹分析显示，LonHS mRNA在肺、十二指肠、肝脏和心脏中表达，而在胸腺细胞中未表达。
Overlapping cDNA clones containing mRNA for a putative Lon protease (LonHS) were isolated from cDNA libraries prepared from human brain poly(A)(+) RNA. The determined nucleotide sequence contains a 2814-bp open reading frame with two potential initiation codons (positions 62-64 and 338-340). The 5'-terminal 337-nucleotide fragment of LonHS mRNA is highly enriched with G and C nucleotides and could direct synthesis of the LonHS N-terminal domain. More likely this region promotes initiation of protein synthesis from the second AUG codon in a cap-independent manner. The amino acid sequence initiated at the second AUG codon includes 845 residues, over 30% of which are identical to those of eubacterial Lon proteases. Residues of the 'A' and 'B' motifs of NTP-binding pattern and a plausible catalytic serine residue are conserved in LonHS. Northern blot analysis revealed LonHS mRNA in lung, duodenum, liver and heart, but not in thymus cells.