In vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in Kenyan women
In vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in Kenyan women
复制标题
DOI：
10.1016/j.anaerobe.2009.11.002
复制
发表时间：
2010-06-01
期刊：
影响因子：
2.3
通讯作者：
Bukusi, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Matu, Martin N.;Orinda, George O.;Bukusi, Elizabeth A.
乳酸杆菌已被证明可以抑制许多病原体的体外生长，并已被用作益生菌来治疗多种胃肠道和/或阴道疾病。我们试图确定阴道来源的乳杆菌对一些与细菌性阴道病 (BV) 相关的细菌的体外抑制潜力，以表征这些乳杆菌产生的抑制物质并评估 H2O2 的产生。阴道样本是通过在 BV 治疗两个月后对 107 名女性的阴道侧壁进行擦拭而获得的。一百五十八种乳酸杆菌。 107 名女性中有 82 名被隔离。詹氏乳杆菌是这些女性中分离出的主要菌株（29/158；18.4%）。在测试了 158 种培养物上清液对 BV 相关细菌的抗菌活性时，没有一种培养物上清液能够抑制脆弱拟杆菌的生长，而 23% (37/158)、28% (45/158) 和 29% (46/158) 的培养物上清液能够抑制普雷沃氏菌、阴道加德纳菌和动杆菌属的生长。分别。乳酸杆菌产生的上清液的pH范围在2.62和6.71之间；高酸性（pH 2-3.99）上清液对指示菌株的抑制作用更强。对上清液进行化学和物理处理后，平均抑制区显着减少 (p = 0.0025)。酸、细菌素和 H2O2 具有拮抗细菌病原体的潜力。这些物质可能会相互增强，而不是各自独立地对病原体起作用。 (C) 2009 Elsevier Ltd. 保留所有权利。
Lactobacilli have been shown to inhibit in vitro growth of many pathogens and have been used as probiotics to treat a broad range of gastrointestinal and/or vaginal disorders. We sought to determine the in vitro inhibitory potential of lactobacilli of vaginal origin to some bacteria associated with bacterial vaginosis (BV), to characterize the inhibitory substances produced by these lactobacilli and to assess H2O2 production. Vaginal specimens were obtained by swabbing the lateral vaginal walls from 107 women two months following BV treatment. One hundred and fifty eight Lactobacillus spp. were isolated in 82 of the 107 women. Lactobacillus jensenii was the predominant strain isolated among these women (29/158; 18.4%). Among 158 culture supernatants tested for antibacterial activity against BV-associated bacteria, none inhibited the growth of Bacteroides fragilis while 23% (37/158), 28% (45/158) and 29% (46/158) inhibited the growth of Prevotella bivia, Gardnerella vaginalis and Mobiluncus spp. respectively. The lactobacilli produced supernatants with a pH range between 2.62 and 6.71; the highly acidic (pH 2-3.99) supernatants were more inhibitory to the indicator strains. There was significant reduction in the mean zones of inhibition following chemical and physical treatment of the supernatants (p = 0.0025). Acid, bacteriocins and H2O2 demonstrated potential for antagonism of the bacterial pathogens. These substances may augment each other rather that each working independently on the pathogens. (C) 2009 Elsevier Ltd. All rights reserved.