Collybistin, a newly identified brain-specific GEF, induces submembrane clustering of gephyrin
Collybistin, a newly identified brain-specific GEF, induces submembrane clustering of gephyrin
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DOI：
10.1038/71096
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发表时间：
2000-01-01
影响因子：
25
通讯作者：
Kirsch, J
中科院分区：
文献类型：
--
作者：
Kins, S;Betz, H;Kirsch, J
突触后 GABA(A) 和甘氨酸受体簇的形成需要受体相关的外周膜蛋白 gephyrin。在这里，我们描述了一种新型 gephyrin 结合蛋白的两种剪接变体，称为 collybistin I 和 II，它们属于 dbl 样 GDP/GTP 交换因子 (GEF) 家族。 Collybistin II 与 gephyrin 的共表达诱导形成膜下 gephyrin 聚集体，从而积累异源寡聚甘氨酸受体。我们的数据表明，collybistin II 通过激活 Rho/Rac 家族的 GTPase 来调节 gephyrin 的膜沉积。因此，该蛋白可能是抑制突触后膜形成和可塑性的重要决定因素。
The formation of postsynaptic GABA(A) and glycine receptor clusters requires the receptor-associated peripheral membrane protein gephyrin. Here we describe two splice variants of a novel gephyrin-binding protein, termed collybistin I and II, which belong to the family of dbl-like GDP/GTP exchange factors (GEFs). Co-expression of collybistin II with gephyrin induced the formation of submembrane gephyrin aggregates that accumulate hetero-oligomeric glycine receptors. Our data suggest that collybistin II regulates the membrane deposition of gephyrin by activating a GTPase of the Rho/Rac family. Therefore, this protein may be an important determinant of inhibitory postsynaptic membrane formation and plasticity.