Gut microbiome alterations in patients with stage 4 hepatitis C
Gut microbiome alterations in patients with stage 4 hepatitis C
复制标题
DOI：
10.1186/s13099-016-0124-2
复制
发表时间：
2016-09-13
期刊：
影响因子：
4.2
通讯作者：
Aziz, Ramy K.
中科院分区：
文献类型：
--
作者：
Aly, AbdelRahman Mahmoud;Adel, AbdelReheem;Aziz, Ramy K.
背景资料：丙型肝炎病毒（HCV）引起使人衰弱的肝脏疾病，其可能进展为肝硬化和癌症，并且在全世界每年夺去50万人的生命。虽然HCV流行病学，病理生理学和治疗正在深入研究，但很少关注HCV感染，HCV诱导的慢性肝病和人类肠道微生物之间的相互作用。由于埃及有世界上最高的流行率的HCV感染，我们启动了这项研究，以监测差异的肠道微生物群落组成的埃及HCV患者，可能会影响，或导致，患者的肝脏states.Results：为此，我们分析了粪便样本从6个阶段4-HCV患者和8个健康人的高通量16 S rRNA基因测序使用Illumina MiSeq。总体而言，健康人肠道微生物组的α多样性高于HCV患者。然而，拟杆菌门的成员在HCV患者中更丰富，健康个体具有更高丰度的厚壁菌门、变形菌门和放线菌门。属水平分析显示普雷沃氏菌属和粪杆菌属（在HCV患者中较高）与瘤胃球菌属和梭菌属（健康组）的丰度差异，表明HCV患者中拟杆菌属的较高丰度最可能是由于普雷沃氏菌属过量。益生菌属，双歧杆菌，只观察到在微生物的健康individuals.Conclusions：据我们所知，这项研究提供了第一个概述的主要门和属区分阶段4-HCV患者从健康个体，并建议可能的微生物组重塑慢性丙型肝炎，可能形成的细菌易位，以及肝脏的消化和蛋白质合成受损的作用。未来的研究将调查更多患者的微生物组组成和功能能力，同时追踪一些潜在的生物标志物分类群（例如，普雷沃氏菌属、粪杆菌属与双歧杆菌属）。
Background: Hepatitis C virus (HCV) causes debilitating liver diseases, which may progress to cirrhosis and cancer, and claims 500,000 annual lives worldwide. While HCV epidemiology, pathophysiology, and therapy are being deeply studied, rare attention is given to reciprocal interactions between HCV infection, HCV-induced chronic liver diseases, and the human gut microbiome. As Egypt has the world's highest prevalence of HCV infections, we launched this study to monitor differences in the gut microbial community composition of Egyptian HCV patients that may affect, or result from, the patients' liver state.Results: To this end, we analyzed stool samples from six stage 4-HCV patients and eight healthy individuals by high-throughput 16S rRNA gene sequencing using Illumina MiSeq. Overall, the alpha-diversity of the healthy persons' gut microbiomes was higher than those of the HCV patients. Whereas members of phylum Bacteroidetes were more abundant in HCV patients, healthy individuals had higher abundance of Firmicutes, Proteobacteria, and Actinobacteria. Genus-level analysis showed differential abundance of Prevotella and Faecalibacterium (higher in HCV patients) vs. Ruminococcus and Clostridium (healthy group), indicating that the higher abundance of Bacteroidetes in HCV patients is most likely due to Prevotella overabundance. The probiotic genus, Bifidobacterium, was only observed in the microbiotas of healthy individuals.Conclusions: To the best of our knowledge, this study provides a first overview of major phyla and genera differentiating stage 4-HCV patients from healthy individuals and suggests possible microbiome remodeling in chronic hepatitis C, possibly shaped by bacterial translocation as well as the liver's impaired role in digestion and protein synthesis. Future studies will investigate the microbiome composition and functional capabilities in more patients while tracing some potential biomarker taxa (e.g., Prevotella, Faecalibacterium vs. Bifidobacterium).