Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography
Imaging the interaction of roots and phosphate fertiliser granules using 4D X-ray tomography
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DOI：
10.1007/s11104-015-2425-5
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发表时间：
2016-04-01
期刊：
影响因子：
4.9
通讯作者：
Roose, Tiina
中科院分区：
文献类型：
--
作者：
Ahmed, Sharif;Klassen, Trudy Naugler;Roose, Tiina
植物根系构型适应土壤环境和养分的分布。许多物种对肥料颗粒附近的局部高营养供应区域作出反应，提高这些区域的分枝密度。然而，对这些适应性的观察通常限于在理想材料中培养的植物（例如，水凝胶），其具有结构少，均匀的矩阵，这是空间上的限制，并在根观察的情况下，只提供2D数据，不完全quantitative.Methods在这项研究中，在体内，时间分辨，微焦点X射线CT成像（μ CT）在3D被用来可视化，量化和评估根/肥料的相互作用，小麦植物在农业土壤中的整个植物生命周期。两种对比肥料[三重过磷酸钙（TSP）和鸟粪石（Crystal GreenA（R））]根据3种不同的处理施用，每种处理为每株植物提供相当于80 kg P2 O 5 ha（-1）的磷（仅鸟粪石，仅TSP和50：50的混合物）。mu CT扫描（60 μ m的空间分辨率）的植物根获得超过14 weeks.Results这是第一次，在原位根/土壤/肥料的相互作用已经可视化的3D从植物发芽到成熟。结果表明，侧根往往通过几毫米的磷（P）源。在这个长度尺度上，根能够访问的P扩散从granulation.Conclusions根/肥料相互作用的定量分析表明，生根密度与颗粒体积损失的缓释，鸟粪石肥料。
Aims Plant root system architecture adapts to the prevailing soil environment and the distribution of nutrients. Many species respond to localised regions of high nutrient supply, found in the vicinity of fertiliser granules, by elevating branching density in these areas. However, observation of these adaptations is frequently limited to plants cultured in idealised materials (e.g., hydrogels) which have a structure-less, homogenous matrix, which are spatially limited and in the case of rhizotron observation provide only 2D data that are not fully quantitative.Methods In this study, in vivo, time resolved, micro-focus X-ray CT imaging (mu CT) in 3D was used to visualise, quantify and assess root/fertiliser interactions of wheat plants in an agricultural soil during the entire plant life cycle. Two contrasting fertilisers [Triple superphosphate (TSP) and struvite (Crystal GreenA (R))] were applied according to 3 different treatments, each providing an equivalent of 80 kg P2O5 ha(-1) (struvite only, TSP only and a 50:50 mixture) to each plant. mu CT scans (60 mu m spatial resolution) of the plant roots were obtained over 14 weeks.Results This is the first time that in situ root/soil/fertiliser interactions have been visualised in 3D from plant germination through to maturity. Results show that lateral roots tend to pass within a few millimetres of the phosphorus (P) source. At this length scale, roots are able to access the P diffusing from the granule.Conclusions Quantitative analysis of root/fertiliser interactions has shown that rooting density correlates with granule volume-loss for a slow release, struvite fertiliser.