Quantitative Evaluation of Adhesion of Osteosarcoma Cells to Hydrophobic Polymer Substrate with Tunable Elasticity
Quantitative Evaluation of Adhesion of Osteosarcoma Cells to Hydrophobic Polymer Substrate with Tunable Elasticity
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DOI：
10.1021/jp212385p
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发表时间：
2012-07-19
影响因子：
3.3
通讯作者：
Tanaka, Motomu
中科院分区：
文献类型：
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作者：
Yoshikawa, Hiroshi Y.;Cui, Jing;Tanaka, Motomu
我们研究了疏水性聚丙烯酸正丁酯网络（cPnBA）作为具有可调弹性的基质在培养、维持和调控人骨肉瘤细胞（U2OS）方面的潜在应用。利用原子力显微镜进行的纳米压痕实验表明，cPnBA薄膜的机械性能在水性条件下得以保持，这证实了基质弹性可以简单地通过交联程度来控制，而与培养基无关。我们发现，通过氧等离子体和血清蛋白等表面处理，可以提高U2OS细胞对cPnBA基质的粘附性。为了确定细胞粘附的强度，利用强皮秒激光脉冲引发的冲击波测量了使细胞从cPnBA基质上脱离的临界压力。细胞粘附强度随基质弹性单调增加，这表明本征疏水性的cPnBA作为一类新型基质材料具有潜力，其具有不受培养基影响的可调机械性能。
We investigated a potential application of hydrophobic poly(n-butyl acrylate) networks (cPnBA) as substrates with tunable elasticity for culturing, maintenance, and regulation of human osteosarcoma cells (U2OS). Nanoindentation experiments with an atomic force microscope revealed that the mechanical properties of cPnBA films are maintained under aqueous conditions, confirming that the substrate elasticity can be controlled simply by the degree of cross-linking, independent from the culture medium. We found that the adhesion U2OS cells to cPnBA substrates could be improved by surface treatments such as oxgen plasma and serum proteins. To determine the strength of cell adhesion, the critical pressure to detach cells from cPnBA substrates was measured using a shock wave induced by an intensive picosecond laser pulse. A monotonic increase in the cell adhesion strength in accordance with the substrate elasticity demonstrated the potential of intrinsically hydrophobic cPnBA as a new class of substrate material with tunable mechanical properties that are not influenced by the culture medium.