Evolution of multi-functional capillary electrophoresis for high-efficiency selection of aptamers
Evolution of multi-functional capillary electrophoresis for high-efficiency selection of aptamers
复制标题
多功能毛细管电泳适配体高效选择的进展
DOI：
10.1016/j.biotechadv.2019.107432
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发表时间：
2019
影响因子：
16
通讯作者：
Feng Qu
中科院分区：
文献类型：
--
作者：
Chao Zhu;Ge Yang;Ghulam Murtaza;Linsen Li;Feng Qu
适配子作为一种新兴的分子识别元件，在临床诊断、药物输送、治疗、环境监测和食品安全分析等方面受到了广泛的关注。适体作为体外筛选抗体类似物，能够识别与抗体相似甚至优于抗体的高亲和力和特异性的各种靶标。然而，选择的限制和低效一直阻碍着它们的更广泛应用。在各种改进的指数富集法(SELEX)中，毛细管电泳法(CE)-SELEX具有多种功能和优势，具有强大的定性和定量分析能力、较少的样品和分析试剂消耗、自然的结合环境、较高的筛选效率和多种模式的可用性。这篇综述总结了领先研究小组在CE-SELEX领域的关键发展，包括我们团队十年的研究和经验，以帮助研究人员充分了解和利用CE-SELEX。简要介绍了适配子的历史、应用以及SELEX的研究进展，重点介绍了CE-SELEX方法与传统SELEX方法相比的优点。此外，我们描述了一些基本的CE-SELEX模型，并提供了CE-SELEX的概述，包括目标和单链DNA文库，选择过程中的每个技术点，以及SELEX后协议。我们期待这一综述将启发更多的研究人员从CE-SELEX的角度对筛选问题有更深入的了解，并将有助于提高选择效率和成功概率，以满足生物分析和医学领域日益增长的适体发现需求。
Aptamers have drawn considerable attention as newly emerging molecular recognition elements in clinical diagnostics, drug delivery, therapeutics, environmental monitoring, and food safety analyses. As the in vitro screening antibody analogs, aptamers are enabled to recognize various types of targets with high affinity and specificity like or even superior to antibodies. However, the restrictions and inefficiency of selection have been hampering their wider application. Among various modified systematic evolution of ligands by exponential enrichment (SELEX) methods, capillary electrophoresis (CE)-SELEX holds multiple functions and advantages with the powerful qualitative and quantitative analysis capabilities, less consumption of sample and analytical reagent, natural binding environment, higher screening efficiency, and availability in multiple modes. This review summarizes the key developments in the area of CE-SELEX by leading research groups, including our teams' ten years of research and experience to help researchers fully understand and utilize CE-SELEX. Aptamers' history, applications, as well as the SELEX developments, have been briefly described; the advantages of CE-SELEX are highlighted compared with the conventional SELEX methods. Further, we describe some essential CE-SELEX models and provide an overview of the CE-SELEX, including the targets and ssDNA library, every technical point in the selection process, and post-SELEX protocol. We expect this review will inspire more researchers to have insight into the screening problems from CE-SELEX viewpoint and will help to improve the selection efficiency and probability of success to meet the growing needs of aptamers' discovery in bioanalytical and medical fields.