ATP binding cassette transporters ABCG1 and ABCG16 affect reproductive development via auxin signalling in Arabidopsis
ATP binding cassette transporters ABCG1 and ABCG16 affect reproductive development via auxin signalling in Arabidopsis
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ATP 结合盒转运蛋白 ABCG1 和 ABCG16 通过生长素信号传导影响拟南芥的生殖发育
DOI：
10.1111/tpj.14690
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发表时间：
2020-02-17
期刊：
影响因子：
7.2
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
--
作者：
Liu, Liping;Zhao, Lihua;Qin, Yuan
被子植物的生殖发育是一个复杂的过程，包括花器官的发育、雄配子体的形成和雌雄生殖器官之间的相互作用以实现成功受精。以往的研究已经揭示了ATP结合盒G亚家族(ABCG)转运蛋白Abcg1和ABCG16在花粉发育过程中的冗余功能，但它们是否参与其他生殖过程尚不清楚。结果表明，Abcg1和ABCG16不仅在花药和雄蕊花丝中表达，而且在柱头、花柱、传输道和胚珠等雌蕊组织中也有丰富的表达。我们进一步证明，雌蕊表达的Abcg1和ABCG16通过影响雌蕊中生长素的分布和生长素流动来促进花粉管的快速生长。此外，雄蕊花丝生长素动态平衡的破坏与花丝伸长缺陷有关。我们的工作揭示了Abcg1和ABCG16在生殖发育中的关键功能，并为鉴定拟南芥中的Abcg1和ABCG16底物提供了线索。
Angiosperm reproductive development is a complex event that includes floral organ development, male and female gametophyte formation and interaction between the male and female reproductive organs for successful fertilization. Previous studies have revealed the redundant function of ATP binding cassette subfamily G (ABCG) transporters ABCG1 and ABCG16 in pollen development, but whether they are involved in other reproductive processes is unknown. Here we show that ABCG1 and ABCG16 were not only expressed in anthers and stamen filaments but also enriched in pistil tissues, including the stigma, style, transmitting tract and ovule. We further demonstrated that pistil-expressed ABCG1 and ABCG16 promoted rapid pollen tube growth through their effects on auxin distribution and auxin flow in the pistil. Moreover, disrupted auxin homeostasis in stamen filaments was associated with defective filament elongation. Our work reveals the key functions of ABCG1 and ABCG16 in reproductive development and provides clues for identifying ABCG1 and ABCG16 substrates in Arabidopsis.