MEASUREMENT OF THE TRANSIT-TIME FOR CELLS THROUGH THE EPIDERMIS AND STRATUM-CORNEUM OF THE MOUSE AND GUINEA-PIG
MEASUREMENT OF THE TRANSIT-TIME FOR CELLS THROUGH THE EPIDERMIS AND STRATUM-CORNEUM OF THE MOUSE AND GUINEA-PIG
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DOI：
10.1111/j.1365-2184.1987.tb01355.x
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发表时间：
1987-09-01
期刊：
影响因子：
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通讯作者：
MAIBACH, HI
中科院分区：
文献类型：
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作者：
POTTEN, CS;SAFFHILL, R;MAIBACH, HI
提出了一种确定通过表皮的传递时间的新方法，包括温和洗涤皮肤表面以收集松散附着的表面角质层。据信，这将比胶带剥离或刮擦更不可能刺激系统。放射性标记的胸腺嘧啶和碘脱氧尿嘧啶已被用于标记基底层的细胞，各种标记的氨基酸（甘氨酸、胱氨酸和蛋氨酸）已被用于标记代谢活性细胞层（直至并包括颗粒层）。由此产生的表面放射性水平的变化已被解释为提供无毛和有毛小鼠表皮从基础到表面的传递时间为8-9.5天，豚鼠为13.5天。小鼠到颗粒层的传递时间（可能包括一些基底层停留时间）约为4.5 d，豚鼠约为8 d。小鼠从颗粒到表面的时间约为5天。结果还表明，当核和细胞器在颗粒层中降解时，释放的物质可以通过角质层迅速扩散到表面。其中一些可以通过色谱法证明是胸腺嘧啶。因此，角质层先于核苷等分子。在注射[125I]-碘脱氧尿嘧啶后不久，也可以检测到这种通过皮肤向外的快速扩散。
A new approach to determine the transit time through the epidermis is presented, involving a gentle washing of the skin surface to collect the loosely attached surface corneocytes. This, it is believed, will be less likely to stimulate the system than tape-stripping or scraping. Radioactivity labelled thymidine and iododeoxyuridine have been used to label cells in the basal layer and various labelled amino acids (glycine, cystine and methionine) have been used to label the metabolically viable cell layers (up to and including the granular layer). The resulting changes in surface radioactivity levels have been interpreted to provide a basal to surface transit time of 8-9.5 days for hairless and haired mouse epidermis and about 13.5 days for guinea-pigs. The basal to granular layer transit time, which probably includes some basal layer residence time, is about 4.5 days in the mouse and 8 days in the guinea-pig. The granular to surface time in mice is about 5 days. The results also suggest that when nuclear and cytoplasmic organelles are degraded in the granular layer, material is released that can diffuse rapidly through the stratum corneum to the surface. Some of this can be shown by chromatography to be thymidine. Hence, the stratum corneum is previous to molecules such as nucleosides. This rapid diffusion outwards through the skin can also be detected shortly after injecting [125I]-iododeoxyuridine.