REQUIREMENTS AND FUNCTIONS OF VESICULAR STOMATITIS VIRUS-L AND NS PROTEINS IN THE TRANSCRIPTION PROCESS INVITRO
REQUIREMENTS AND FUNCTIONS OF VESICULAR STOMATITIS VIRUS-L AND NS PROTEINS IN THE TRANSCRIPTION PROCESS INVITRO
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DOI：
10.1016/0006-291x(85)90568-6
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
BANERJEE, AK
中科院分区：
文献类型：
--
作者：
DE, BP;BANERJEE, AK
从水泡性口炎病毒（VSV）的核糖核蛋白复合物（RNAP）中分离纯化了L蛋白和NS蛋白，并研究了它们在体外RNA合成重建过程中的需要和功能。L和NS蛋白的最佳RNA合成的要求被发现是催化和化学计量，分别。随着NS蛋白加入量的增加和L蛋白的饱和，N-mRNA与前导RNA的合成比例呈线性增加。相反，当L蛋白的浓度增加时，相应的比率保持恒定。这些结果，再加上观察到的L蛋白参与RNA合成的起始，表明NS蛋白参与RNA链的延伸步骤。NS蛋白可能与L蛋白和模板N-RNA相互作用，并将后者解旋以促进L蛋白在模板RNA上的移动。
The L and NS proteins of vesicular stomatitis virus were purified from transcribing ribonucleoprotein complex and were used to study their requirements and functions during reconstitution of RNA synthesis in vitro. The requirements for L and NS proteins for optimal RNA synthesis were found to be catalytic and stoichiometric, respectively. Addition of increasing amounts of NS protein to N-RNA template and saturating L protein, the ratio of N-mRNA to leader RNA synthesis increased linearly. In contrast, when the concentration of L protein was increased the corresponding ratio remained constant. These results, coupled with the observation that the L protein is involved in the initiation of RNA synthesis, suggest that the NS protein is involved in the RNA chain elongation step. The NS protein possibly interacts with both the L protein and the template N-RNA and unwinds the latter to facilitate the movement of L protein on the template RNA.