The oculomotor internuclear pathway: a double retrograde labeling study.
The oculomotor internuclear pathway: a double retrograde labeling study.
复制标题
动眼神经核间通路：双重逆行标记研究。
DOI：
10.1016/0006-8993(83)90464-x
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Phipps,BS
中科院分区：
文献类型：
--
作者：
Maciewicz,R;Phipps,BS
为确定动眼神经核间神经元(INOS)与外展神经核(NVI)建立单侧或双侧联系，将辣根过氧化物酶(HRP)、DAPI或核黄(NY)注射到NVI，并对动眼神经复合体(NII)进行逆行标记细胞的检查。这种注射导致中脑导水管周围灰质腹侧NII内和背侧有大量的iNOS逆行标记；大约10%的HRP阳性细胞也被荧光标记。在实验的条件下，这种双重标记的频率在很大程度上与注射的荧光标记的HRP的量或标记在注射部位的铺展程度无关。它也与使用哪种荧光色素作为第二标记无关。单个双标记细胞散在分布于单标iNOS内，并直接背向NiII。这些发现为向NVI有双侧投射的NiII iNOS的子集提供了强有力的证据；然而，双重标记的低频率表明，投射到两个NVI的iNOS的数量可能很少。
To determine whether oculomotor internuclear neurons (INOs) establish unilateral or bilateral connections with the abducens nucleus (nVI), horseradish peroxidase (HRP) and either DAPI or Nuclear Yellow (NY) were injected into nVI and the oculomotor complex (nIII) examined for retrogradely labeled cells. Such injections resulted in retrograde labeling of a large number of INOs within and just dorsal to nIII in the ventral periaqueductal gray; approximately 10% of HRP-positive cells were also labeled with a fluorescent marker. Within the conditions of the experiment, this frequency of double-labeling was largely independent of the amount of HRP of fluorescent label injected or the degree of spread of label at the injections sites. It was also independent of which fluorochrome was used as a second label. Individual double-labeled cells were scattered within and immediately dorsal to nIII within the distribution of single-labeled INOs. These findings provide strong evidence for a subset of nIII INOs that have bilateral projections to nVI; the low frequency of double-labeling, however, suggests that the population of INOs projecting to both nVI may be small.