Thin-Layer Preparations of Dithiothreitol-Treated Bronchial Washing Specimens
Thin-Layer Preparations of Dithiothreitol-Treated Bronchial Washing Specimens
复制标题
二硫苏糖醇处理的支气管冲洗标本的薄层制备
DOI：
10.1159/000326581
复制
发表时间：
2003
期刊：
影响因子：
1.8
通讯作者：
P. Shield
中科院分区：
文献类型：
--
作者：
Kirsti I. Koivurinne;P. Shield
目的
为了评价二硫苏糖醇（DTT）处理和ThinPrep（TP）（Cytyc Corp，Boxborough，马萨诸塞州，美国）的联合作用，支气管冲洗标本的处理。
研究设计
共431支气管冲洗标本最初用溶于30%甲醇溶液的0.05% DTT处理。离心后，制备1个TP载玻片和2-4个常规细胞离心涂片或涂片制备物（CP）。在所有情况下，对两种制剂的报告进行比较。还比较了所有48例异常病例和52例连续阴性病例的细胞组成、细胞分布、易于判读和总体制备质量。记录了20例病例的筛选时间。
结果
一个TP载玻片的诊断准确性似乎与2-4个CP的诊断准确性相当。在100例配对标本中，85%的TP载玻片的总体质量被评估为等于或上级于CP。TP载玻片的背景更干净，细胞面积更小，显著缩短了筛选时间。粘液溶解和标本均质化并不总是最佳的，偶尔会导致不均匀的二次采样和细胞TP差。然而，总体而言，认为TP玻片的总体质量优于CP玻片上级。
结论
标本质量的提高和筛选时间的减少必须与TP技术的高消耗品成本相平衡。
OBJECTIVE
To evaluate the combined effect of dithiothreitol (DTT) treatment and ThinPrep (TP) (Cytyc Corp, Boxborough, Massachusetts, U.S.A.) processing on bronchial washing specimens.
STUDY DESIGN
A total of 431 bronchial washing specimens were initially treated with 0.05% DTT in a 30% methanol solution. After centrifugation, 1 TP slide and 2-4 conventional cytospin or smear preparations (CPs) were prepared. The reports of both preparations were compared in all cases. All 48 abnormal cases and 52 consecutive negative cases were also compared for cellular composition, distribution of the cells, ease of interpretation and overall preparation quality. Screening time was recorded for 20 of the cases.
RESULTS
The diagnostic accuracy of one TP slide appeared comparable to that of 2-4 CPs. The TP slide was assessed to be equal or superior in overall quality to CP in 85% of 100 cases of paired specimens. The cleaner background and smaller cellular area of TP slides significantly reduced the screening time. Mucolysis and specimen homogenization were not always optimal, occasionally resulting in uneven subsampling and poorly cellular TPs. However, in general, TP slides were considered superior to CPs in overall quality.
CONCLUSION
Improvement in specimen quality and reduced screening time have to be balanced against the high cost of consumables with the TP technique.
将痰细胞与粘液糖蛋白安全分离。
DOI：
--
发表时间：
1995
期刊：
Acta cytologica.
影响因子：
--
作者：
Tockman,MS;Qiao,Y;Li,L;Zhao,GZ;Sharma,R;Cavenaugh,LL;Erozan,YS
通讯作者：
Erozan,YS