Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata
Characterization of a novel arsenite long-distance transporter from arsenic hyperaccumulator fern Pteris vittata
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砷超富集植物蕨 Pteris vittata 中新型亚砷酸盐长距离转运蛋白的表征
DOI：
10.1111/nph.17962
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发表时间：
2022-01-30
期刊：
影响因子：
9.4
通讯作者：
He，Zhenyan
中科院分区：
文献类型：
--
作者：
Yan，Huili;Xu，Wenxiu;He，Zhenyan
马齿蕨是砷超富集植物，地上生物量可积累数千mg As kg−1DW。其超积累能力的一个关键因素是其高效的As长距离转运系统。本研究通过构建马铃薯PvAsE 1的全长cDNA过表达文库，分离到PvAsE 1，并通过酵母系统、RNA干扰配子体和孢子体、亚细胞定位和原位杂交等方法对其进行了鉴定。PvAsE 1是一种面向质膜的砷（AsIII）外排剂。PvAsE 1的沉默减少了AsIII在P. vittata孢子体中的长距离转运。PvAsE 1在结构上类似于溶质载体（SLC）13蛋白。在根木质部周围的薄壁组织细胞中可以观察到其转录本。估计出现时间为atc。52.7 Ma.PvAsE1是一种以前未表征的SLC 13样AsIII外排剂，可能有助于通过木质部负载进行AsIII长距离转运。PvAsE 1在蕨类植物演化中出现较晚，可能是在古近纪-古近纪边界对选择压力的适应主体。PvAsE 1的鉴定为揭示蜈蚣藻特殊的砷超富集特性提供了线索。
Pteris vittatais an arsenic (As) hyperaccumulator that can accumulate several thousand mg As kg−1DW in aboveground biomass. A key factor for its hyperaccumulation ability is its highly efficient As long‐distance translocation system. However, the underlying molecular mechanisms remain unknown.We isolatedPvAsE1through the full‐length cDNA over‐expression library ofP. vittataand characterized it through a yeast system, RNAi gametophytes and sporophytes, subcellular‐location andin situhybridization. Phylogenomic analysis was conducted to estimate the appearance time of PvAsE1.PvAsE1 was a plasma membrane‐oriented arsenite (AsIII) effluxer. The silencing ofPvAsE1reduced AsIII long‐distance translocation inP. vittatasporophytes. PvAsE1 was structurally similar to solute carrier (SLC)13 proteins. Its transcripts could be observed in parenchyma cells surrounding the xylem of roots. The appearance time was estimated atc. 52.7 Ma.PvAsE1 was a previously uncharacterized SLC13‐like AsIII effluxer, which may contribute to AsIII long‐distance translocation via xylem loading. PvAsE1 appeared late in fern evolution and might be an adaptive subject to the selection pressure at the Cretaceaou–Paleogene boundary. The identification of PvAsE1 provides clues for revealing the special As hyperaccumulation characteristics ofP. vittata.