Inferring the antigenic epitopes for highly pathogenic avian influenza H5N1 viruses
Inferring the antigenic epitopes for highly pathogenic avian influenza H5N1 viruses
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高致病性禽流感H5N1病毒抗原表位的推断
DOI：
10.1016/j.vaccine.2013.12.005
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发表时间：
2014-02-03
期刊：
影响因子：
5.5
通讯作者：
Jiang, Taijiao
中科院分区：
文献类型：
--
作者：
Peng, Yousong;Zou, Yuanqiang;Jiang, Taijiao
从宿主免疫监测中逃避流感病毒的主要是通过其表面蛋白血凝素（HA）介导的，这是流感疫苗的主要成分。因此，鉴定流感病毒抗原表位对HA的鉴定不仅可以帮助我们了解病毒免疫逃逸的分子机制，而且还促进了疫苗菌株的选择。尽管以前的努力，但缺乏对高致病性禽流感（HPAI）H5N1病毒的抗原表位的系统定义。在这项研究中，我们通过整合根据人类流感H3N2病毒的HA映射的抗原部位来推断H5N1病毒的HA抗原表位，据报道，这些位点与H5病毒中的免疫逃逸和突变热点位点相关的部位在HPAI H5N1 VIRUSES HPAI HPAI H5N1 VIRUSES的进化史中鉴定出来。我们表明，这些推断的抗原表位在HPAI H5N1病毒的抗原变异中起着重要作用。基于推断的抗原表位，我们进一步开发了一种计算方法，可以有效预测HPAI H5N1病毒的抗原变异型（可在http://biocloud.hhnu.edu.edu.edu.edu.edu.edu.cn/predict/html/html/index.html）。因此，我们的工作不仅推断了HPAI H5N1病毒的抗原表位，而且还提供了一种有效的计算方法来协助疫苗建议保护致命的鸟类流感。 （c）2013 Elsevier Ltd.保留所有权利。
The evasion of influenza virus from host immune surveillance is mainly mediated through its surface protein hemagglutinin (HA), the main component of influenza vaccine. Thus, identification of influenza virus antigenic epitopes on HA can not only help us understand the molecular mechanisms of viral immune escape but also facilitate vaccine strain selection. Despite previous efforts, there is a lack of systematic definition of the antigenic epitopes for the highly pathogenic avian influenza (HPAI) H5N1 viruses. In this study, we infer the HA antigenic epitopes for H5N1 viruses by integrating the antigenic sites mapped from the HA of human influenza H3N2 viruses, the sites which were reported to be associated with immune escape in H5 viruses and the mutation hotspot sites identified in the evolutionary history of HPAI H5N1 viruses. We show that these inferred antigenic epitopes play significant roles in antigenic variation of HPAI H5N1 viruses. Based on inferred antigenic epitopes, we further develop a computational method to effectively predict antigenic variants for HPAI H5N1 viruses (available at http://biocloud.hnu.edu.cn/predict/html/index.html). Therefore, our work has not only inferred the antigenic epitopes for HPAI H5N1 viruses but also provided an effective computational method to assist vaccine recommendations for protection against the deadly bird flu. (C) 2013 Elsevier Ltd. All rights reserved.