A STUDY ON THE MECHANISM OF DNA EXCRETION FROM P-AERUGINOSA KYU-1 - EFFECT OF MITOMYCIN-C ON EXTRACELLULAR DNA PRODUCTION
A STUDY ON THE MECHANISM OF DNA EXCRETION FROM P-AERUGINOSA KYU-1 - EFFECT OF MITOMYCIN-C ON EXTRACELLULAR DNA PRODUCTION
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DOI：
10.1080/00021369.1981.10864927
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
UEDA, S
中科院分区：
文献类型：
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作者：
HARA, T;UEDA, S
利用KYU-1菌株研究了DNA分泌的机制。该菌株在细胞外产生大量的DNA（7.8mg/ml），并且细胞外DNA/ml培养物的量是细胞内DNA/ml培养物的量的几十倍。通过添加抑制剂（萘啶酸、5-氟尿嘧啶或丝裂霉素C）抑制DNA合成;其抑制率为35- 54%。在对数生长期中期加入100 μ g/ml丝裂霉素C，仅获得0.89mg细胞外DNA/ml培养物;抑制率为95%。在培养物中合成的Pi的量在有或没有抑制剂的情况下几乎相同。电镜观察到KYU-1菌株分泌的DNA为双链DNA。
The mechanism of excretion of DNA was investigated using strain KYU-1. This strain produced a considerable amount of DNA extracellularly (7.8 mg/ml) and the amount of extracellular DNA/ml of the culture was scores of times more compared to that of intracellular DNA/ml of the culture. DNA synthesis was repressed by the addition of inhibitors (nalidixic acid, 5-fluorouracil or mitomycin C); their inhibition rates were 35-54%. With the addition of 100 .mu.g/ml of mitomycin C at the middle of the logarithmic growth phase, only 0.89 mg of extracellular DNA/ml of the culture was obtained; the inhibition rate was 95%. The amount of Pi synthesized in the culture was almost the same with or without an inhibitor. The excretion of DNA fron the cell surface of strain KYU-1 was observed on EM and the DNA molecules excreted were double-stranded.