Molecular cloning of a functional bovine interleukin 2 cDNA.
Molecular cloning of a functional bovine interleukin 2 cDNA.
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功能性牛白细胞介素 2 cDNA 的分子克隆。
DOI：
10.1073/pnas.83.10.3228
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发表时间：
1986
影响因子：
11.1
通讯作者：
Magnuson,JA
中科院分区：
文献类型：
--
作者：
Reeves,R;Spies,AG;Nissen,MS;Buck,CD;Weinberg,AD;Barr,PJ;Magnuson,NS;Magnuson,JA
牛白细胞介素 2 (IL-2) 基因的 cDNA 克隆已被分离出来，并被证明在转染至猴细胞后可产生分泌型牛 IL-2 蛋白。牛IL-2克隆长791个碱基对，包含474个碱基对的开放阅读框，编码158个氨基酸的牛IL-2前体多肽，估计分子量为17,884。前体蛋白的推定疏水前导序列或信号序列长23个氨基酸残基，表明通过加工去除后，成熟分泌的牛IL-2蛋白含有135个氨基酸，分子量为15,464。将牛 IL-2 的核苷酸序列和预测的氨基酸序列与人和小鼠 IL-2 的核苷酸序列和预测氨基酸序列进行比较，显示物种之间存在广泛的序列保守区域，其中散布着其他相似性较低的区域。牛 IL-2 基因的 3' 非翻译区与人类和小鼠基因的 3' 非翻译区具有同样多（如果不是更高）的序列同源性，就像这些基因的转录编码区一样，表明该区域参与调节。特别是，在牛 IL-2 克隆的 3' 非翻译尾中发现的串联重复序列 (TATT)n 也在其他已知的白细胞介素和干扰素基因的 3' 非翻译区以及淋巴和免疫反应系统的许多其他诱导基因的类似区域中发现，表明这些进化上保守的序列具有细胞或组织特异性调节功能。
A cDNA clone of the bovine interleukin 2 (IL-2) gene has been isolated and demonstrated to be functional in the production of secreted bovine IL-2 protein when transfected into monkey cells. The bovine IL-2 clone is 791 base pairs in length and contains an open reading frame of 474 base pairs coding for a bovine IL-2 precursor polypeptide of 158 amino acids with an estimated molecular weight of 17,884. The putative hydrophobic leader or signal sequence of the precursor protein is 23 amino acid residues long, suggesting that, after removal by processing, the mature secreted bovine IL-2 protein contains 135 amino acids and has a molecular weight of 15,464. Comparisons of both the nucleotide sequence and the predicted amino acid sequence of bovine IL-2 with those of the human and mouse IL-2 show extensive regions of sequence conservation between the species, interspersed with other regions of less similarity. The 3' untranslated region of the bovine IL-2 gene shares as much, if not greater, sequence homology with the 3' untranslated regions of the human and mouse genes as do the transcribed coding regions of these genes, suggesting an involvement of this region in regulation. In particular, a tandemly repeated sequence, (TATT)n, found in the 3' untranslated tail of the bovine IL-2 clone is also found in the 3' untranslated region of the other known interleukin and interferon genes, as well as in similar regions of many other inducible genes of the lymphoid and immune response systems, suggesting a cell or tissue-specific regulatory function for these evolutionarily conserved sequences.