IL-15, in synergy with RAE-1ε, stimulates TCR-independent proliferation and activation of CD8+ T cells
IL-15, in synergy with RAE-1ε, stimulates TCR-independent proliferation and activation of CD8+ T cells
复制标题
DOI：
10.3892/ol.2011.495
复制
发表时间：
2012-02-01
期刊：
影响因子：
2.9
通讯作者：
Tian, Fang
中科院分区：
文献类型：
--
作者：
Qian, Li;Zhang, Yue;Tian, Fang
CD 8（+）T细胞通过杀死恶性或病毒感染的细胞在免疫监视中发挥关键作用。白细胞介素15（IL-15）是促进CD 8（+）T细胞的增殖和效应能力的关键细胞因子，并且已被用于支持肿瘤疾病的细胞疗法中的CD 8（+）T细胞的生长。最近的研究表明，IL-15与其他细胞因子如IL-6协同作用，增强了CD 8（+）T细胞的T细胞受体（TCR）非依赖性增殖和功能。本研究的目的是研究BaF 3-mb 15-RAE细胞在刺激小鼠CD 8（+）T细胞中的作用。在DMEM中培养BaF 3细胞并生长B16 F10细胞。MTT法检测CD 8（+）T细胞增殖情况。使用流式细胞术分析细胞。结果显示，IL-15与另一种T细胞刺激分子RAE 1 β协同作用，在不存在通过TCR的信号传导的情况下，有效地促进CD 8（+）T细胞的增殖，诱导CD 8（+）T细胞活化并增强颗粒酶B和干扰素-γ（IFN-γ）的产生。此外，IL-15与RAE 1 β的组合导致对CD 8（+）T细胞介导的针对B16 F10肿瘤细胞的细胞毒性的协同效应。因此，本研究的结果表明，IL-15，协同RAE 1 β，在体外增强CD 8（+）T细胞的TCR非依赖性效应功能，这可能是有用的癌症的细胞免疫治疗。
CD8(+)T cells play critical roles in immunosurveillance by killing malignant or virally infected cells. Interleukin 15 (IL-15) is a critical cytokine for promoting proliferation and the effector capacity of CD8(+) T cells, and has been used to support the growth of CD8(+) T cells in cellular therapies of neoplastic diseases. Recent studies have shown that IL-15, in synergy with other cytokines, such as IL-6, enhances the T-cell receptor (TCR)-independent proliferation and function of CD8(+) T cells. The aim of the present study was to investigate the role of BaF3-mb15-RAE cells in stimulating mouse CD8(+) T cells. BaF3 cells were cultured and B16F10 cells were grown in DMEM. MTT assay was used to detect the proliferation of CD8(+) T cells. Cells were analyzed using flow cytometry. The results showed that IL-15 synergistically acts with another T-cell stimulatory molecule, RAE1 epsilon, to potently promote the proliferation of CD8(+) T cells, induce CD8(+) T-cell activation and enhance granzyme B and interferon-gamma (IFN-gamma) production in the absence of signaling via the TCR. Moreover, IL-15 in combination with RAE1 epsilon resulted in a cooperative effect on CD8(+) T-cell-mediated cytotoxicity against B16F10 tumor cells. Thus, results of the present study showed that IL-15, in synergy with RAE1 epsilon, enhances the TCR-independent effector function of CD8(+) T cells in vitro, which may be useful in the cellular immunotherapy of cancer.