CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms.
CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms.
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DOI：
10.4049/jimmunol.161.4.1710
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发表时间：
1998-08
影响因子：
4.4
通讯作者：
Masahiko Amano;Nicole Baumgarth;M. Dick;L. Brossay;Mitchell Kronenberg;Leonard A. Herzenberg;S. Strober
中科院分区：
文献类型：
--
作者：
Masahiko Amano;Nicole Baumgarth;M. Dick;L. Brossay;Mitchell Kronenberg;Leonard A. Herzenberg;S. Strober
我们已经使用了流式细胞仪分析，免疫组织学，和功能测定，以研究正常和β 2 m-/-小鼠的B细胞亚群的CD 1的表达。在正常小鼠中鉴定出两种表达高水平CD 1的B细胞亚群：脾边缘区B细胞（IgM高、IgD低、CD 21高、CD 24中、CD 23-CD 43-）和新鉴定的滤泡B细胞亚群。后一种细胞是不寻常的，因为它们与滤泡B细胞一样是IgD高CD 23+，但表达高水平的CD 21和IgM，这是一种与边缘区B细胞相关的表达模式。因此，发现CD 1和CD 21的高水平表达与脾B细胞密切相关。免疫组织学证实边缘区B细胞和脾滤泡中的B细胞簇上表达CD 1。在β 2 m-/-小鼠中，这些细胞的高水平CD 1表达和脾脏、淋巴结、腹膜腔和骨髓中B细胞亚群的低水平CD 1表达均显著降低。尽管如此，CD 1限制性T细胞克隆在对从β 2 m-/-和野生型小鼠获得的LPS激活的脾细胞的反应中剧烈增殖。这种反应被3C 11抗CD 1 mAb抑制。这些结果显示了B细胞亚群在表达β 2 m依赖性形式的CD 1方面的异质性。他们进一步表明，CD 1的β 2 m非依赖性形式在可刺激T细胞的B细胞上表达;然而，这种形式在本文使用的抗CD 1 mAb中不易观察到。
We have used multicolor FACS analysis, immunohistology, and functional assays to study the expression of CD1 on B cell subsets from normal and beta 2m-/- mice. Two B cell subpopulations were identified that express high levels of CD1 in normal mice: splenic marginal zone B cells (IgMhigh IgDlow CD21high CD24intermediate CD23- CD43-) and a newly identified subpopulation of follicular B cells. The latter cells are unusual, because they are IgDhigh CD23+, like follicular B cells, but express high levels of CD21 and IgM, an expression pattern that is associated with marginal zone B cells. Therefore, the high-level expression of CD1 and CD21 was found to be closely associated on splenic B cells. Immunohistology confirmed the expression of CD1 on marginal zone B cells and on clusters of B cells in splenic follicles. Both the high-level CD1 expression by these cells and the low-level CD1 expression by subpopulations of B cells in the spleen, lymph node, peritoneal cavity, and bone marrow were markedly reduced in beta 2m-/- mice. Despite this, a CD1-restricted T cell clone proliferated vigorously in response to LPS-activated spleen cells that had been obtained from both beta 2m-/- and wild-type mice. This response was inhibited by the 3C11 anti-CD1 mAb. These results show the heterogeneity of B cell subsets in their expression of the beta 2m-dependent form of CD1. They further suggest that a beta 2m-independent form of CD1 is expressed on B cells that can stimulate T cells; however, this form is not easily visualized with the anti-CD1 mAb used here.