Arabidopsis <i>CURLY LEAF</i> functions in leaf immunity against fungal pathogens by concomitantly repressing <i>SEPALLATA3</i> and activating <i>ORA59</i>
Arabidopsis <i>CURLY LEAF</i> functions in leaf immunity against fungal pathogens by concomitantly repressing <i>SEPALLATA3</i> and activating <i>ORA59</i>
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拟南芥<i>CURLY LEAF</i>通过同时抑制<i>SEPALLATA3</i>和激活<i>ORA59</i>来发挥叶对真菌病原体的免疫功能
DOI：
10.1111/tpj.15488
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Takano Yoshitaka
中科院分区：
文献类型：
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作者：
Singkaravanit‐Ogawa Suthitar;Kosaka Ayumi;Kitakura Saeko;Uchida Kotaro;Nishiuchi Takumi;Ono Erika;Fukunaga Satoshi;Takano Yoshitaka
拟南芥对包括炭疽菌在内的非适应真菌病原体的非寄主抗性包括入侵前和入侵后的免疫反应。在此，我们报道了非寄主对非适应性炭疽病的抗性。拟南芥叶片需要CURLY叶(CLF)，它对叶片的发育、开花和生长至关重要。对病原体行为的微观分析表明，CLFin在入侵前和入侵后的非宿主抗性中都是必需的。SEP3基因的缺失不仅抑制了植物生长和叶片发育的缺陷，而且还抑制了非寄主对非适应热带炭疽菌的抗性。然而，SEP3在拟南芥野生型叶片中的异位过表达并没有破坏非寄主抗性。参与非寄主抗性的多种植物防御素(PDF)基因的表达。热带植物在树叶中被压抑。此外，PDF表达所需的拟南芥ORA59基因也在叶片中受到抑制。值得注意的是，当SEP3在理论59突变背景中过表达时，热带棉铃虫在接种的叶片中产生了明显的损伤，表明非寄主抗性受到了损害。此外，高表达SEP3的59株植物对适应的炭疽病菌的叶片免疫力存在缺陷。由于理论上59株高表达SEP3的植株没有表现出明显的卷叶或生长减慢，与突变体相反，这些结果强烈表明，与CLF在生长和叶片发育中的作用无关，拟南芥叶片对炭疽菌的免疫需要伴随着SEP3的抑制和通过CLF诱导的ORA59。
Arabidopsis non‐host resistance against non‐adapted fungal pathogens includingColletotrichumfungi consists of pre‐invasive and post‐invasive immune responses. Here we report that non‐host resistance against non‐adaptedColletotrichumspp. in Arabidopsis leaves requiresCURLY LEAF(CLF), which is critical for leaf development, flowering and growth. Microscopic analysis of pathogen behavior revealed a requirement forCLFin both pre‐ and post‐invasive non‐host resistance. The loss of a functionalSEPALLATA3(SEP3) gene, ectopically expressed inclfmutant leaves, suppressed not only the defect of theclfplants in growth and leaf development but also a defect in non‐host resistance against the non‐adaptedColletotrichum tropicale. However, the ectopic overexpression ofSEP3in Arabidopsis wild‐type leaves did not disrupt the non‐host resistance. The expression of multiple plant defensin (PDF) genes that are involved in non‐host resistance againstC. tropicalewas repressed inclfleaves. Moreover, theOctadecanoid‐responsive Arabidopsis 59(ORA59) gene, which is required forPDFexpression, was also repressed inclfleaves. Notably, whenSEP3was overexpressed in theora59mutant background,C. tropicaleproduced clear lesions in the inoculated leaves, indicating an impairment in non‐host resistance. Furthermore,ora59plants overexpressingSEP3exhibited a defect in leaf immunity to the adaptedColletotrichum higginsianum. Since theora59plants overexpressingSEP3did not display obvious leaf curling or reduced growth, in contrast to theclfmutants, these results strongly suggest that concomitantSEP3repression andORA59induction via CLF are required for Arabidopsis leaf immunity toColletotrichumfungi, uncoupled from CLF’s function in growth and leaf development.