The HSP/co-chaperone network in environmental cold adaptation of Chilo suppressalis
The HSP/co-chaperone network in environmental cold adaptation of Chilo suppressalis
复制标题
二化螟环境冷适应的热休克蛋白/共伴侣网络
DOI：
10.1016/j.ijbiomac.2021.07.113
复制
发表时间：
2021-08-04
影响因子：
8.2
通讯作者：
Lin, Yongjun
中科院分区：
文献类型：
--
作者：
Jiang, Fan;Chang, Guofeng;Lin, Yongjun
冬季寒冷是外温动物主要的环境胁迫之一。二化螟是一种危害水稻的鳞翅目害虫，其分布范围广，包括环境条件恶劣的温带地区。虽然C.二化螟具有出色的耐寒性，但其冷适应机制尚不清楚。在这里，我们使用生物信息学的方法来评估转录水平的基因组成的C。二化螟热休克蛋白（HSP）/辅伴侣网络响应冷诱导的压力。利用C. suppressalis基因组的转录水平，我们实验研究了冷驯化或间歇冷休克胁迫下滞育和非滞育幼虫相应的转录水平。我们共鉴定了19个HSP和8个HSP共伴侣蛋白。suppressalis基因组其中9个（hsp 90、hsp 75、hsp 70、hsp 40、small hsp、90 kDa热休克蛋白ATP酶样激活物、热休克因子、热休克因子结合蛋白1样和HSPB 1相关蛋白1）是高度冷诱导的，可能构成了C. suppressalis.我们还发现，转录调控的HSP/辅伴侣网络反应之间的冷驯化和短期冷休克不同。此外，HSP/辅伴侣网络的激活依赖于越冬幼虫的滞育状态，冷驯化可能进一步提高幼虫的耐冷性。这些研究结果为深入了解C. suppressalis.
Winter cold is one of the major environmental stresses for ectotherm species. Chilo suppressalis, a notorious lepidopteran pest of rice, has a wide geographic region that includes temperate zones with severe environmental conditions. Although C. suppressalis exhibits remarkable cold tolerance, its cold-adaptation mechanisms remain unclear. Here, we used bioinformatics approaches to evaluate transcript levels of genes comprising the C. suppressalis heat shock protein (HSP)/co-chaperone network in response to cold-induced stress. Using all such genes identified in the C. suppressalis genome, we experimentally examined the corresponding transcript levels under cold-acclimation or intermittent cold-shock stresses in diapause and non-diapausing larvae. In total, we identified 19 HSPs and 8 HSP co-chaperones in the C. suppressalis genome. Nine (hsp90, hsp75, hsp70, hsp40, small hsp, activator of 90 kDa heat shock protein ATPase-like, heat shock factor, heat shock factor binding protein 1-like and HSPB1-associated protein 1) were highly cold-inducible and likely comprise the principal cold-response HSP/cochaperone network in C. suppressalis. We also found that transcriptional regulation of the HSP/co-chaperone networks response differs between cold-acclimation and short-term cold-shock. Moreover, activation of the HSP/co-chaperone network depends on the diapause state of overwintering larvae and cold acclimation may further increase larval cold tolerance. These results provide key new insights in the cold-adaptation mechanisms in C. suppressalis.