Tumor marker-responsive behavior of gels prepared by biomolecular imprinting
Tumor marker-responsive behavior of gels prepared by biomolecular imprinting
复制标题
DOI：
10.1073/pnas.0506786103
复制
发表时间：
2006-01-31
影响因子：
11.1
通讯作者：
Uragami, T
中科院分区：
文献类型：
--
作者：
Miyata, T;Jige, M;Uragami, T
我们报告动态糖蛋白识别的凝胶制备的生物分子印迹使用凝集素和抗体分子作为配体的肿瘤特异性标记糖蛋白。用微量交联剂制备的糖蛋白印迹凝胶可以通过凝集素和抗体配体动态识别肿瘤特异性标记糖蛋白，并根据糖蛋白浓度诱导体积变化。糖蛋白印迹凝胶收缩响应于一个目标糖蛋白，但nonimprinted凝胶溶胀一点。印迹凝胶的糖蛋白响应性收缩是由凝集素-糖蛋白-抗体复合物的形成引起的，该复合物充当可逆交联点。只有当凝胶中的凝集素和抗体同时识别目标糖蛋白的糖链和肽链时，糖蛋白印迹凝胶才会收缩。由于生物分子印迹凝胶响应于糖蛋白的收缩行为使得能够准确地检测和识别肿瘤特异性标记糖蛋白，因此它们在传感系统和分子诊断中作为智能设备具有许多潜在的应用。
We report dynamic glycoprotein recognition of gels prepared by biomolecular imprinting using lectin and antibody molecules as ligands for tumor-specific marker glycoproteins. The glycoprotein-imprinted gels prepared with minute amounts of cross-linkers could dynamically recognize tumor-specific marker glycoproteins by lectin and antibody ligands and induce volume changes according to the glycoprotein concentration. The glycoprotein-imprinted gel shrank in response to a target glycoprotein but nonimprinted gel swelled a little. The glycoprotein-responsive shrinking of the imprinted gel was caused by formation of lectin-glycoprotein-antibody complexes that acted as reversible cross-linking points. Glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. As shrinking behavior of biomolecularly imprinted gels in response to glycoproteins enables the accurate detection and recognition of tumor-specific marker glycoproteins, they have many potential applications as smart devices in sensing systems and for molecular diagnostics.