Overall bacterial community composition and abundance of nitrifiers and denitrifiers in a typical macrotidal estuary
Overall bacterial community composition and abundance of nitrifiers and denitrifiers in a typical macrotidal estuary
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典型大潮汐河口的总体细菌群落组成以及硝化细菌和反硝化细菌的丰度
DOI：
10.1016/j.marpolbul.2017.09.062
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发表时间：
2018
影响因子：
5.8
通讯作者：
Wu Weixiang
中科院分区：
文献类型：
--
作者：
Zhu Weijing;Wang Cheng;Sun Faqian;Zhao Liancheng;Dou Wenjie;Mao Zhihua;Wu Weixiang
耦合的氮循环过程可以缓解河口生态系统中过量氮负荷引起的富营养化的负面影响。研究了杭州湾沉积物中硝化细菌和硝化细菌在不同环境梯度上的丰度和多样性。定量PCR和Pearson相关分析表明，细菌氨氧化菌（AOB）是具有氨氧化能力的优势菌群，而非S编码菌在反硝化过程中占优势。同时，亚硝酸盐和pH值也是影响微生物和细菌丰度以及细菌群落分布的两个主要因素。nirS/AOBamoA基因丰度比与亚硝酸盐浓度呈负相关。荧光原位杂交进一步证实了AOB和乙酸盐还原细胞在沉积物中紧密相连并形成明显的聚集体。这些结果为杭州湾沉积物中硝化-反硝化耦合过程提供了初步的认识。
Coupled nitrogen cycling processes can alleviate the negative effects of eutrophication caused by excessive nitrogen load in estuarine ecosystems. The abundance and diversity of nitrifiers and denitrifiers across different environmental gradients were examined in the sediment of Hangzhou Bay. Quantitative PCR and Pearson's correlation analyses suggested that the bacterial ammonia-oxidizers (AOB) were the dominant phylotypes capable of ammonia oxidation, while thenirS-encoding denitrifiers predominated in the denitrification process. Simultaneously, nitrite and pH were found to be the two major factors influencingamoAandnirgene abundances, and the distribution of bacterial communities. Moreover, the ratio ofnirS/AOBamoAgene abundance showed negative correlation with nitrite concentration. Fluorescencein situhybridization further demonstrated that AOB and acetate-denitrifying cells were closely connected and formed obvious aggregates in the sediment. Together, all these results provided us a preliminary insight for coupled nitrification-denitrification processes in the sediment of Hangzhou Bay.