Retinal Tissue Oxygen Tension Imaging in the Rat
Retinal Tissue Oxygen Tension Imaging in the Rat
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DOI：
10.1167/iovs.09-4710
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发表时间：
2010-09-01
影响因子：
4.4
通讯作者：
Wu, Tingting
中科院分区：
文献类型：
--
作者：
Shahidi, Mahnaz;Wanek, Justin;Wu, Tingting
目的.目的：建立一种测量视网膜组织氧分压（PO（2））的成像技术，并探讨通过调节吸入氧分数（FiO（2））来测量大鼠视网膜PO（2）变化的可行性。一条狭窄的激光线以一定角度投射在视网膜上，在玻璃体内注射氧敏感分子探针后，磷光发射被成像。一个频域的方法被用于磷光寿命测量。视网膜PO（2）图由磷光寿命图像计算，通过大鼠在10%、21%和50%FiO（2）条件下的视网膜深度得出氧分布图。视网膜PO（2）的测量是可重复的，并且沿图像沿着不同位置的视网膜外层和内层PO（2）的变化不显著（P >= 0.3）。在10%、21%和50%FiO（2）中获得的最大外视网膜PO（2）有显著差异（P < 0.0001）。视网膜外膜最大PO（2）与体动脉PO（2）相关（R = 0.70; P < 0.0001）。视网膜外层PO（2）曲线的斜率与视网膜外层PO（2）最大值相关（R = 0.84; P < 0.0001）。视网膜内层PO（2）平均值与视网膜外层PO（2）最大值相关（R = 0.88; P < 0.0001）。已经开发了一种用于定量绘制视网膜组织氧分压的技术，其具有能够连续监测健康和疾病中的视网膜氧合的潜力。（Invest Ophthalmol维斯科学。2010;51：4766 - 4770）DOI：10.1167/iovs.09-4710
PURPOSE. To report an imaging technique for measurement of oxygen tension (PO(2)) in retinal tissue and establish its feasibility for measuring retinal PO(2) variations in rat eyes by adjusting the fraction of inspired oxygen (FiO(2)).METHODS. A narrow laser line was projected at an angle on the retina, and phosphorescence emission was imaged after intra-vitreal injection of an oxygen-sensitive molecular probe. A frequency-domain approach was used for phosphorescence lifetime measurements. Retinal PO(2) maps were computed from phosphorescence lifetime images, and oxygen profiles through the retinal depth were derived in rats in conditions of 10%, 21%, and 50% FiO(2).RESULTS. Retinal PO(2) measurements were repeatable, and variations in outer and inner retina PO(2) at different locations along the image were not significant (P >= 0.3). Maximum outer retinal PO(2) obtained in 10%, 21%, and 50% FiO(2) were significantly different (P < 0.0001). Maximum outer retinal PO(2) correlated with systemic arterial PO(2) (R = 0.70; P < 0.0001). The slope of the outer retina PO(2) profile correlated with maximum outer retinal PO(2) (R = 0.84; P < 0.0001). Mean inner retina PO(2) correlated with maximum outer retinal PO(2) (R = 0.88; P < 0.0001).CONCLUSIONS. A technique has been developed for quantitative mapping of retinal tissue oxygen tension with the potential to enable sequential monitoring of retinal oxygenation in health and disease. (Invest Ophthalmol Vis Sci. 2010;51:4766 - 4770) DOI:10.1167/iovs.09-4710